Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator
Journal Article
·
· Acta Crystallographica. Section F
- Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras (Portugal)
The sorbitol operon regulator from K. pneumoniae has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 3.2 Å. The sorbitol operon regulator (SorC) regulates the metabolism of l-sorbose in Klebsiella pneumonia. SorC was overexpressed in Escherichia coli and purified, and crystals were obtained of a tetrameric form. A single crystal showed X-ray diffraction to 3.20 Å. The crystal belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 91.6, b = 113.3, c = 184.1 Å. Analysis of the molecular-replacement solution indicates the presence of four SorC molecules in the asymmetric unit.
- OSTI ID:
- 22360462
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Crystallization and preliminary X-ray characterization of 1,3-propanediol dehydrogenase from the human pathogen Klebsiella pneumoniae
Crystallization and preliminary X-ray studies of SdiA from Escherichia coli
Crystallization of an Lhx3–Isl1 complex
Journal Article
·
Wed Feb 28 23:00:00 EST 2007
· Acta Crystallographica. Section F
·
OSTI ID:22360285
Crystallization and preliminary X-ray studies of SdiA from Escherichia coli
Journal Article
·
Mon Dec 31 23:00:00 EST 2007
· Acta Crystallographica. Section F
·
OSTI ID:22360460
Crystallization of an Lhx3–Isl1 complex
Journal Article
·
Tue Apr 01 00:00:00 EDT 2008
· Acta Crystallographica. Section F
·
OSTI ID:22360507