Crystallization and preliminary X-ray studies of SdiA from Escherichia coli
Journal Article
·
· Acta Crystallographica. Section F
E. coli SdiA was overexpressed, purified and crystallized. The crystals belonged to the hexagonal space group P6{sub 1}22 or P6{sub 5}22 and diffracted to 2.7 Å resolution. SdiA enhances cell division by regulating the ftsQAZ operon in Escherichia coli as a transcription activator. In addition, SdiA is suggested to play a role in detecting quorum signals that emanate from other species. It is therefore a homologue of LuxR, a cognate quorum-sensing receptor that recognizes a quorum signal and activates the quorum responses. To elucidate the role of SdiA and its functional and structural relationship to LuxR, structural studies were performed on E. coli SdiA. Recombinant SdiA was overexpressed, purified and crystallized at 287 K using the hanging-drop vapour-diffusion method. X-ray diffraction data from a native crystal were collected with 99.7% completeness to 2.7 Å resolution with an R{sub merge} of 6.0%. The crystals belong to the hexagonal space group P6{sub 1}22 or P6{sub 5}22, with unit-cell parameters a = b = 130.47, c = 125.23 Å.
- OSTI ID:
- 22360460
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis
Preparation, crystallization and preliminary X-ray characterization of a conserved hypothetical protein XC1692 from Xanthomonas campestris
Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of pyrrolysyl-tRNA synthetase from the methanogenic archaeon Methanosarcina mazei
Journal Article
·
Fri Jul 01 00:00:00 EDT 2005
· Acta Crystallographica. Section F
·
OSTI ID:22356148
Preparation, crystallization and preliminary X-ray characterization of a conserved hypothetical protein XC1692 from Xanthomonas campestris
Journal Article
·
Fri Jul 01 00:00:00 EDT 2005
· Acta Crystallographica. Section F
·
OSTI ID:22356154
Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of pyrrolysyl-tRNA synthetase from the methanogenic archaeon Methanosarcina mazei
Journal Article
·
Sun Oct 01 00:00:00 EDT 2006
· Acta Crystallographica. Section F
·
OSTI ID:22356363