Crystallization and preliminary X-ray diffraction analysis of the full-length Mg{sup 2+} transporter MgtE

Journal Article · Wed Aug 01 04:00:00 EDT 2007 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309107032332· OSTI ID:22360394

Hattori, Motoyuki; Tanaka, Yoshiki ^[1]; Fukai, Shuya ^[2]; Ishitani, Ryuichiro ^[1]; Nureki, Osamu ^[1]

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1. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501 (Japan)
2. Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501 (Japan)

Selenomethionine-substituted crystals of the Mg{sup 2+} transporter MgtE from T. thermophilus diffracted to 3.5 Å resolution and belonged to space group C222{sub 1}, with unit-cell parameters a = 118.3, b = 134.9, c = 366.2 Å. The MgtE family of Mg{sup 2+} transporters are ubiquitously conserved in all three domains. The genes encoding full-length MgtE from seven different species were cloned. Three of the seven MgtE transporters were overexpressed and purified for use in crystallization trials. Only Thermus thermophilus MgtE was successfully crystallized using the sitting-drop vapour-diffusion method. Selenomethionine-substituted (SeMet) crystals were obtained by cross-microseeding using the native microcrystals. The SeMet crystals diffracted X-rays to 3.5 Å resolution using synchrotron radiation and belong to space group C222{sub 1}, with unit-cell parameters a = 118.3, b = 134.9, c = 366.2 Å. Structure determination is in progress.

• OSTI ID: 22360394
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 8 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIFFUSION
LENGTH
LIPIDS
MAGNESIUM
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION
X-RAY DIFFRACTION