Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg{sup 2+} transporter MgtE

Journal Article · Wed Aug 01 04:00:00 EDT 2007 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309107032344· OSTI ID:22360391

Tanaka, Yoshiki; Hattori, Motoyuki ^[1]; Fukai, Shuya ^[2]; Ishitania, Ryuichiro ^[1]; Nureki, Osamu ^[1]

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1. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501 (Japan)
2. Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501 (Japan)

Crystals of the cytosolic domain of the Mg{sup 2+} transporter MgtE from T. thermophilus in the presence and absence of Mg{sup 2+} diffracted X-rays to 2.3 and 3.5 Å resolution, respectively, and belong to space groups P6{sub 5}22 (a = b = 57.7, c = 317.6 Å) and P2{sub 1}2{sub 1}2{sub 1} (a = 77.0, b = 100.3, c = 100.3 Å), respectively. The MgtE family of Mg{sup 2+} transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg{sup 2+} transporters include a cystathionine-β-synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg{sup 2+}. The crystals formed in the presence of Mg{sup 2+} diffracted X-rays to 2.3 Å resolution using synchrotron radiation, belong to space group P6{sub 5}22 with unit-cell parameters a = b = 57.7, c = 317.6 Å and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg{sup 2+} diffracted X-rays to 3.5 Å resolution using synchrotron radiation, belong to space group P2{sub 1}2{sub 1}2{sub 1} with unit-cell parameters a = 77.0, b = 100.3, c = 100.3 Å and are expected to contain two molecules in the asymmetric unit.

• OSTI ID: 22360391
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 8 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
MAGNESIUM
MOLECULES
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION
X-RAY DIFFRACTION