Purification, crystallization and preliminary X-ray analysis of Mycobacterium tuberculosis folylpolyglutamate synthase (MtbFPGS)

Journal Article · Thu Jun 01 00:00:00 EDT 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309106017180· OSTI ID:22360220

Smith, C. A. ^[1]; Sun, X. ^[2]; Baker, E. N.; Metcalf, P. ^[3]

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1. Stanford Synchrotron Radiation Laboratory, MS 992575 Sand Hill Road, Menlo Park, CA 94025 (United States)
2. HortResearch Palmerston North, Tennent Drive, Private Bag 11 030, Palmerston North (New Zealand)
3. Laboratory of Structural Biology, School of Biological Sciences, Thomas Building, 3A Symonds Street, Auckland (New Zealand)

The gene encoding M. tuberculosis FPGS (MtbFPGS; Rv2447c) has been cloned and the protein has been expressed in E. coli. The purified protein was crystallized and X-ray diffraction data were collected to 2.0 Å resolution. The gene encoding Mycobacterium tuberculosis FPGS (MtbFPGS; Rv2447c) has been cloned and the protein (51 kDa) expressed in Escherichia coli. The purified protein was crystallized either by the batch method in the presence of adenosine diphosphate (ADP) and CoCl{sub 2} or by vapour diffusion in the presence of ADP, dihydrofolate and CaCl{sub 2}. X-ray diffraction data to approximately 2.0 and 2.6 Å resolution were collected at the Stanford Synchrotron Radiation Laboratory (SSRL) for crystals grown under the respective conditions. Both crystals belong to the cubic space group P2{sub 1}3, with a unit-cell parameter of 112.6 and 111.8 Å, respectively. Structure determination is proceeding.

• OSTI ID: 22360220
• Journal Information: Acta Crystallographica. Section F, Vol. 62, Issue Pt 6; Other Information: PMCID: PMC2243083; PMID: 16754987; PUBLISHER-ID: bw5148; OAI: oai:pubmedcentral.nih.gov:2243083; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIFFUSION
ESCHERICHIA COLI
PROTEINS
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION
X-RAY DIFFRACTION