Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv2827c from Mycobacterium tuberculosis

Journal Article · Tue Aug 01 00:00:00 EDT 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309106024213· OSTI ID:22360184

Janowski, Robert ^[1]; Nasser Eddine, Ali; Kaufmann, Stefan H. E. ^[2]

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1. EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg (Germany)
2. Max-Planck-Institute for Infection Biology, Schumannstrasse 21/22, D-10117 Berlin (Germany)

M. tuberculosis hypothetical protein Rv2827c was cloned, expressed, purified and crystallized. Preliminary X-ray diffraction data were collected to a resolution of 1.93 Å. The hypothetical protein Rv2827c from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. It was purified using affinity and size-exclusion chromatographic techniques and then crystallized. Preliminary X-ray diffraction data analysis suggests the presence of two translationally related molecules in the asymmetric unit of the orthorhombic crystals.

• OSTI ID: 22360184
• Journal Information: Acta Crystallographica. Section F, Vol. 62, Issue Pt 8; Other Information: PMCID: PMC2242928; PMID: 16880548; PUBLISHER-ID: ll5066; OAI: oai:pubmedcentral.nih.gov:2242928; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
AFFINITY
CRYSTALLIZATION
CRYSTALS
DATA ANALYSIS
ESCHERICHIA COLI
MOLECULES
PROTEINS
RESOLUTION
X-RAY DIFFRACTION