Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p

Journal Article · Wed Mar 01 04:00:00 EST 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309106004702· OSTI ID:22356308

Wu, Yunkun ^[1]; McCombs, Debbie; Nagy, Lisa; DeLucas, Lawrence ^[2]; Sha, Bingdong ^[1]

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1. Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham (United States)
2. Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham (United States)

Tom70p is an important translocase of the outer membrane complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) has been crystallized. Protein translocations across mitochondrial membranes play critical roles in mitochondrion biogenesis. Protein transport from the cell cytosol to the mitochondrial matrix is carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tom70p is an important TOM-complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) was crystallized. The crystals diffract to 3.2 Å using a synchrotron X-ray source and belong to space group P2{sub 1}, with unit-cell parameters a = 44.89, b = 168.78, c = 83.41 Å, α = 90.00, β = 102.74, γ = 90.00°. There are two Tom70p molecules in one asymmetric unit, which corresponds to a solvent content of approximately 51%. Structure determination by MAD methods is under way.

• OSTI ID: 22356308
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 3 Vol. 62; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULES
PRECURSOR
RECEPTORS
SOLVENTS
SPACE GROUPS
SURFACES
SYNCHROTRONS
TRANSLOCATION
X-RAY SOURCES