From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing

Wan, Yao; Hopper, Anita K.

doi:10.1101/gad.316257.118

From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing

Journal Article · Tue Sep 18 00:00:00 EDT 2018 · Genes & Development

DOI:https://doi.org/10.1101/gad.316257.118· OSTI ID:1560569

Wan, Yao ^[1]; Hopper, Anita K. ^[1]

The Ohio State Univ., Columbus, OH (United States)

The mitochondrial cytoplasmic surface serves as a processing site for numerous RNAs from budding yeast to metazoans. We report that budding yeast mitochondrial outer membrane (MOM) proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and Tom 22) and sorting and assembly machinery (Sam37) are required for efficient pretransfer RNA (pre-tRNA) splicing. Defective pre-tRNA splicing in MOM mutants is due not to loss of respiratory metabolism but instead inefficient targeting/tethering of tRNA splicing endonuclease (SEN) subunits to mitochondria. Schizosaccharomyces pombe SEN subunits also localize to mitochondria, and Tom70 is required for this localization and pre-tRNA splicing. Thus, the role of MOM protein in targeting/tethering SEN subunits to mitochondria has been conserved for >500 million years.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1560569

Journal Information:: Genes & Development, Journal Name: Genes & Development Journal Issue: 19-20 Vol. 32; ISSN 0890-9369

Publisher:: Cold Springs Harbor PressCopyright Statement

Country of Publication:: United States

Language:: English

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