The design of macromolecular crystallography diffraction experiments

Journal Article · Fri Apr 01 04:00:00 EDT 2011 · Acta Crystallographica. Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S0907444911007608· OSTI ID:22347920

Evans, Gwyndaf; Axford, Danny; Owen, Robin L. ^[1]

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1. Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE (United Kingdom)

Thoughts about the decisions made in designing macromolecular X-ray crystallography experiments at synchrotron beamlines are presented. The measurement of X-ray diffraction data from macromolecular crystals for the purpose of structure determination is the convergence of two processes: the preparation of diffraction-quality crystal samples on the one hand and the construction and optimization of an X-ray beamline and end station on the other. Like sample preparation, a macromolecular crystallography beamline is geared to obtaining the best possible diffraction measurements from crystals provided by the synchrotron user. This paper describes the thoughts behind an experiment that fully exploits both the sample and the beamline and how these map into everyday decisions that users can and should make when visiting a beamline with their most precious crystals.

• OSTI ID: 22347920
• Journal Information: Acta Crystallographica. Section D: Biological Crystallography, Journal Name: Acta Crystallographica. Section D: Biological Crystallography Journal Issue: Pt 4 Vol. 67; ISSN ABCRE6; ISSN 0907-4449
• Country of Publication: Denmark
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CONVERGENCE
CRYSTALLOGRAPHY
DESIGN
MOLECULAR CRYSTALS
OPTIMIZATION
SAMPLE PREPARATION
SYNCHROTRON RADIATION
SYNCHROTRONS
X-RAY DIFFRACTION