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Title: Optimal fine ϕ-slicing for single-photon-counting pixel detectors

Abstract

Fine ϕ-slicing substantially improves scaling statistics and anomalous signal for diffraction data collection with hybrid pixel detectors. The data-collection parameters used in a macromolecular diffraction experiment have a strong impact on data quality. A careful choice of parameters leads to better data and can make the difference between success and failure in phasing attempts, and will also result in a more accurate atomic model. The selection of parameters has to account for the application of the data in various phasing methods or high-resolution refinement. Furthermore, experimental factors such as crystal characteristics, available experiment time and the properties of the X-ray source and detector have to be considered. For many years, CCD detectors have been the prevalent type of detectors used in macromolecular crystallography. Recently, hybrid pixel X-ray detectors that operate in single-photon-counting mode have become available. These detectors have fundamentally different characteristics compared with CCD detectors and different data-collection strategies should be applied. Fine ϕ-slicing is a strategy that is particularly well suited to hybrid pixel detectors because of the fast readout time and the absence of readout noise. A large number of data sets were systematically collected from crystals of four different proteins in order to investigate the benefitmore » of fine ϕ-slicing on data quality with a noise-free detector. The results show that fine ϕ-slicing can substantially improve scaling statistics and anomalous signal provided that the rotation angle is comparable to half the crystal mosaicity.« less

Authors:
; ;  [1]
  1. Swiss Light Source at Paul Scherrer Institut, CH-5232 Villigen (Switzerland)
Publication Date:
OSTI Identifier:
22347905
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 68; Journal Issue: Pt 1; Other Information: PMCID: PMC3245722; PMID: 22194332; PUBLISHER-ID: wd5161; OAI: oai:pubmedcentral.nih.gov:3245722; Copyright (c) Mueller et al. 2012; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ATOMS; CHARGE-COUPLED DEVICES; CRYSTALS; DIFFRACTION; HYBRIDIZATION; NOISE; PHOTONS; PROTEINS; RESOLUTION; ROTATION; SCALING; SIGNALS; X-RAY SOURCES

Citation Formats

Mueller, Marcus, E-mail: marcus.mueller@dectris.com, Wang, Meitian, and Schulze-Briese, Clemens. Optimal fine ϕ-slicing for single-photon-counting pixel detectors. Denmark: N. p., 2012. Web. doi:10.1107/S0907444911049833.
Mueller, Marcus, E-mail: marcus.mueller@dectris.com, Wang, Meitian, & Schulze-Briese, Clemens. Optimal fine ϕ-slicing for single-photon-counting pixel detectors. Denmark. doi:10.1107/S0907444911049833.
Mueller, Marcus, E-mail: marcus.mueller@dectris.com, Wang, Meitian, and Schulze-Briese, Clemens. Sun . "Optimal fine ϕ-slicing for single-photon-counting pixel detectors". Denmark. doi:10.1107/S0907444911049833.
@article{osti_22347905,
title = {Optimal fine ϕ-slicing for single-photon-counting pixel detectors},
author = {Mueller, Marcus, E-mail: marcus.mueller@dectris.com and Wang, Meitian and Schulze-Briese, Clemens},
abstractNote = {Fine ϕ-slicing substantially improves scaling statistics and anomalous signal for diffraction data collection with hybrid pixel detectors. The data-collection parameters used in a macromolecular diffraction experiment have a strong impact on data quality. A careful choice of parameters leads to better data and can make the difference between success and failure in phasing attempts, and will also result in a more accurate atomic model. The selection of parameters has to account for the application of the data in various phasing methods or high-resolution refinement. Furthermore, experimental factors such as crystal characteristics, available experiment time and the properties of the X-ray source and detector have to be considered. For many years, CCD detectors have been the prevalent type of detectors used in macromolecular crystallography. Recently, hybrid pixel X-ray detectors that operate in single-photon-counting mode have become available. These detectors have fundamentally different characteristics compared with CCD detectors and different data-collection strategies should be applied. Fine ϕ-slicing is a strategy that is particularly well suited to hybrid pixel detectors because of the fast readout time and the absence of readout noise. A large number of data sets were systematically collected from crystals of four different proteins in order to investigate the benefit of fine ϕ-slicing on data quality with a noise-free detector. The results show that fine ϕ-slicing can substantially improve scaling statistics and anomalous signal provided that the rotation angle is comparable to half the crystal mosaicity.},
doi = {10.1107/S0907444911049833},
journal = {Acta Crystallographica. Section D: Biological Crystallography},
number = Pt 1,
volume = 68,
place = {Denmark},
year = {Sun Jan 01 00:00:00 EST 2012},
month = {Sun Jan 01 00:00:00 EST 2012}
}