Imaging Intact Filamentous Fungi from Spore to Hyphal Tip on the Nanoscale

Technical Report · Sat Jul 01 04:00:00 EDT 2023

DOI:https://doi.org/10.2172/2229440· OSTI ID:2229440

Evans, James E. ^[1]; Hill, Eric A. ^[1]; Moser, Trevor H. ^[1]; Brookreson, Jory T. ^[1]

---

1. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Previously, the Pacific Northwest National Laboratory was limited by a 3-order of magnitude gap in resolution for direct 2-D and 3-D imaging of chemical, biological, energy and materials science systems. Our imaging instrumentation could directly visualize 3-D ultrastructure without labeling at resolutions less than 10-nanometers or greater than 10-micrometers. To address this gap, we procured and commissioned a Sigray NanoFast Bio-Lambda laboratory-based x-ray nanotomography system. This equipment provides the capability to image through tens of micrometers of organic materials at down to 35-nanometer resolution to bridge the nanoscale to the mesoscale for biological systems, organic polymers, environmental and particulate samples.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE Laboratory Directed Research and Development (LDRD) Program
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 2229440
• Report Number(s): PNNL--34647
• Country of Publication: United States
• Language: English

Related Subjects

47 OTHER INSTRUMENTATION
59 BASIC BIOLOGICAL SCIENCES