Low-Dose Hyper-Radiosensitivity Is Not a Common Effect in Normal Asynchronous and G2-Phase Fibroblasts of Cancer Patients

Journal Article · Sat Feb 01 04:00:00 EST 2014 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/J.IJROBP.2013.10.031· OSTI ID:22283353

Słonina, Dorota ^[1]; Biesaga, Beata; Janecka, Anna ^[1]; Kabat, Damian ^[2]; Bukowska-Strakova, Karolina ^[3]; Gasińska, Anna ^[1]

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1. Department of Applied Radiobiology, Centre of Oncology, Maria-Skłodowska-Curie Memorial Institute Kraków, Kraków (Poland)
2. Department of Medical Physics, Centre of Oncology, Maria-Skłodowska-Curie Memorial Institute Kraków, Kraków (Poland)
3. Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Kraków (Poland)

Purpose: In our previous study, using the micronucleus assay, a low-dose hyper-radiosensitivity (HRS)-like phenomenon was observed for normal fibroblasts of 2 of the 40 cancer patients investigated. In this article we report, for the first time, the survival response of primary fibroblasts from 25 of these patients to low-dose irradiation and answer the question regarding the effect of G2-phase enrichment on HRS elicitation. Methods and Materials: The clonogenic survival of asynchronous as well as G2-phase enriched fibroblast populations was measured. Separation of G2-phase cells and precise cell counting was performed using a fluorescence-activated cell sorter. Sorted and plated cells were irradiated with single doses (0.1-4 Gy) of 6-MV x-rays. For each patient, at least 4 independent experiments were performed, and the induced-repair model was fitted over the whole data set to confirm the presence of HRS effect. Results: The HRS response was demonstrated for the asynchronous and G2-phase enriched cell populations of 4 patients. For the rest of patients, HRS was not defined in either of the 2 fibroblast populations. Thus, G2-phase enrichment had no effect on HRS elicitation. Conclusions: The fact that low-dose hyper-radiosensitivity is not a common effect in normal human fibroblasts implies that HRS may be of little consequence in late-responding connective tissues with regard to radiation fibrosis.

• OSTI ID: 22283353
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 2 Vol. 88; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
FIBROBLASTS
FIBROSIS
FLUORESCENCE
LOW DOSE IRRADIATION
NEOPLASMS
PATIENTS
RADIOSENSITIVITY