Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane
- Department of Physics, NMSSVN College, Nagamalai, Madurai-625019 (India)
- Department of Bio-functional Science, Kyushu University, Fukuoka (Japan)
- Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka (Japan)
- Department of Chemistry, NMSSVN College, Nagamalai, Madurai-625 019 (India)
Electron spin resonance (ESR) studies were carried out for 2mM {sup 14}N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.
- OSTI ID:
- 22269427
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1591; ISSN APCPCS; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Permeability studies of redox-sensitive nitroxyl spin probes in corn oil using an L-band ESR spectrometer
Electrochemistry and spectroelectrochemistry of nitroxyl free radicals