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Title: Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis

Abstract

Highlights: •G-CSF induces tube formation, migration and proliferation of lymphatic cells. •G-CSF increases phosphorylation of MAPK and Akt in lymphatic endothelial cells. •MAPK and Akt pathways are linked to G-CSF-induced in vitro lymphangiogenesis. •G-CSF increases sprouting of a lymphatic ring. •G-CSF produces peritoneal lymphangiogenesis. -- Abstract: Granulocyte-colony stimulating factor (G-CSF) is reported to induce differentiation in cells of the monocyte lineage and angiogenesis in vascular endothelial cells, but its effects on lymphangiogenesis is uncertain. Here we examined the effects and the mechanisms of G-CSF-induced lymphangiogenesis using human lymphatic endothelial cells (hLECs). Our results showed that G-CSF induced capillary-like tube formation, migration and proliferation of hLECs in a dose- and time-dependent manner and enhanced sprouting of thoracic duct. G-CSF increased phosphorylation of Akt and ERK1/2 in hLECs. Supporting the observations, specific inhibitors of phosphatidylinositol 3′-kinase and MAPK suppressed the G-CSF-induced in vitro lymphangiogenesis and sprouting. Intraperitoneal administration of G-CSF to mice also stimulated peritoneal lymphangiogenesis. These findings suggest that G-CSF is a lymphangiogenic factor.

Authors:
; ; ; ;  [1]; ;  [1];  [2];  [1];  [2];  [1];  [2]
  1. Department of Internal Medicine, Chonbuk National University Medical School, Jeonju (Korea, Republic of)
  2. (Korea, Republic of)
Publication Date:
OSTI Identifier:
22239658
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 436; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANGIOGENESIS; CAPILLARIES; CESIUM FLUORIDES; IN VITRO; LYMPH VESSELS; MICE; MONOCYTES; PHOSPHORYLATION; SPROUTING

Citation Formats

Lee, Ae Sin, Kim, Dal, Wagle, Susbin Raj, Lee, Jung Eun, Jung, Yu Jin, Kang, Kyung Pyo, Lee, Sik, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Park, Sung Kwang, E-mail: parksk@jbnu.ac.kr, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Kim, Won, E-mail: kwon@jbnu.ac.kr, and Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju. Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.05.062.
Lee, Ae Sin, Kim, Dal, Wagle, Susbin Raj, Lee, Jung Eun, Jung, Yu Jin, Kang, Kyung Pyo, Lee, Sik, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Park, Sung Kwang, E-mail: parksk@jbnu.ac.kr, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Kim, Won, E-mail: kwon@jbnu.ac.kr, & Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju. Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis. United States. doi:10.1016/J.BBRC.2013.05.062.
Lee, Ae Sin, Kim, Dal, Wagle, Susbin Raj, Lee, Jung Eun, Jung, Yu Jin, Kang, Kyung Pyo, Lee, Sik, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Park, Sung Kwang, E-mail: parksk@jbnu.ac.kr, Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Kim, Won, E-mail: kwon@jbnu.ac.kr, and Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju. Fri . "Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis". United States. doi:10.1016/J.BBRC.2013.05.062.
@article{osti_22239658,
title = {Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis},
author = {Lee, Ae Sin and Kim, Dal and Wagle, Susbin Raj and Lee, Jung Eun and Jung, Yu Jin and Kang, Kyung Pyo and Lee, Sik and Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju and Park, Sung Kwang, E-mail: parksk@jbnu.ac.kr and Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju and Kim, Won, E-mail: kwon@jbnu.ac.kr and Research Institute of Clinical Medicine of Chonbuk National University-Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju},
abstractNote = {Highlights: •G-CSF induces tube formation, migration and proliferation of lymphatic cells. •G-CSF increases phosphorylation of MAPK and Akt in lymphatic endothelial cells. •MAPK and Akt pathways are linked to G-CSF-induced in vitro lymphangiogenesis. •G-CSF increases sprouting of a lymphatic ring. •G-CSF produces peritoneal lymphangiogenesis. -- Abstract: Granulocyte-colony stimulating factor (G-CSF) is reported to induce differentiation in cells of the monocyte lineage and angiogenesis in vascular endothelial cells, but its effects on lymphangiogenesis is uncertain. Here we examined the effects and the mechanisms of G-CSF-induced lymphangiogenesis using human lymphatic endothelial cells (hLECs). Our results showed that G-CSF induced capillary-like tube formation, migration and proliferation of hLECs in a dose- and time-dependent manner and enhanced sprouting of thoracic duct. G-CSF increased phosphorylation of Akt and ERK1/2 in hLECs. Supporting the observations, specific inhibitors of phosphatidylinositol 3′-kinase and MAPK suppressed the G-CSF-induced in vitro lymphangiogenesis and sprouting. Intraperitoneal administration of G-CSF to mice also stimulated peritoneal lymphangiogenesis. These findings suggest that G-CSF is a lymphangiogenic factor.},
doi = {10.1016/J.BBRC.2013.05.062},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 436,
place = {United States},
year = {Fri Jul 12 00:00:00 EDT 2013},
month = {Fri Jul 12 00:00:00 EDT 2013}
}