Hypoxia-induced mitogenic factor enhances angiogenesis by promoting proliferation and migration of endothelial cells

Journal Article · Wed Nov 01 04:00:00 EST 2006 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2006.07.024· OSTI ID:20858045

Qiangsong, Tong ^[1]; Liduan, Zheng ^[2]; Bo, Li ^[1]; Danming, Wang ^[1]; Chuanshu, Huang ^[3]; Matuschak, George M ^[1]; Dechun, Li ^[1]

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1. Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Saint Louis University, Desloge Towers, 7th Floor, 3635 Vista Avenue, Saint Louis, MO 63110-0250 (United States)
2. Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022 (China)
3. Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987 (United States)

Our previous studies have indicated that hypoxia-induced mitogenic factor (HIMF) has angiogenic properties in an in vivo matrigel plug model and HIMF upregulates expression of vascular endothelial growth factor (VEGF) in mouse lungs and cultured lung epithelial cells. However, whether HIMF exerts angiogenic effects through modulating endothelial cell function remains unknown. In this study, mouse aortic rings cultured with recombinant HIMF protein resulted in enhanced vascular sprouting and increased endothelial cell spreading as confirmed by Dil-Ac-LDL uptake, von Willebrand factor and CD31 staining. In cultured mouse endothelial cell line SVEC 4-10, HIMF dose-dependently enhanced cell proliferation, in vitro migration and tubulogenesis, which was not attenuated by SU1498, a VEGFR2/Flk-1 receptor tyrosine kinase inhibitor. Moreover, HIMF stimulation resulted in phosphorylation of Akt, p38 and ERK1/2 kinases in SVEC 4-10 cells. Treatment of mouse aortic rings and SVEC 4-10 cells with LY294002, but not SB203580, PD098059 or U0126, abolished HIMF-induced vascular sprouting and angiogenic responses. In addition, transfection of a dominant-negative mutant of phosphatidylinositol 3-kinase (PI-3K), {delta}p85, blocked HIMF-induced phosphorylation of Akt, endothelial activation and tubulogenesis. These results indicate that HIMF enhances angiogenesis by promoting proliferation and migration of endothelial cells via activation of the PI-3K/Akt pathways.

• OSTI ID: 20858045
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 18 Vol. 312; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANOXIA
CELL PROLIFERATION
GROWTH FACTORS
IN VITRO
IN VIVO
LUNGS
MICE
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RECEPTORS
SPROUTING
TYROSINE