Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Chemoautotrophic cells comprising an engineered carbon fixation pathway

Patent ·
OSTI ID:2221966
The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Research Organization:
Ginkgo Bioworks, Inc., Boston, MA (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
DOE Contract Number:
AR0000091
Assignee:
Ginkgo Bioworks, Inc. (Boston, MA)
Patent Number(s):
11,697,829
Application Number:
16/943,819
OSTI ID:
2221966
Country of Publication:
United States
Language:
English

References (201)

Characterizing the metabolic phenotype: A phenotype phase plane analysis journal December 2001
Effect of copy number and mRNA processing and stabilization on transcript and protein levels from an engineered dual-gene operon journal April 2002
Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon journal October 2002
An algorithm for operating a fed-batch fermentor at optimum specific-growth rate journal January 1989
Improved NADPH supply for xylitol production by engineered Escherichia coli with glycolytic mutations journal February 2011
A new structure-based classification of sulfide:quinone oxidoreductases journal December 2009
Controlling the Metabolic Flux through the Carotenoid Pathway Using Directed mRNA Processing and Stabilization journal October 2001
Metabolic Engineering through Cofactor Manipulation and Its Effects on Metabolic Flux Redistribution in Escherichia coli journal April 2002
The Effect of NAPRTase Overexpression on the Total Levels of NAD, The NADH/NAD+ Ratio, and the Distribution of Metabolites in Escherichia coli journal July 2002
Overexpression in Escherichia coli of 12 Vitamin B12 Biosynthetic Enzymes journal April 1995
Solubility of Proteins Isolated from Inclusion Bodies Is Enhanced by Fusion to Maltose-Binding Protein or Thioredoxin journal February 1998
A reverse KREBS cycle in photosynthesis: consensus at last journal April 1990
High-cell-density production of poly-β-hydroxybutyrate (PHB) from methanol by Methylobacterium extorquens: production of high-molecular-mass PHB journal December 1995
Determination of saponifiable glycerol in “Bio-Diesel” journal March 1991
Chloroflexus aurantiacus secretes 3-hydroxypropionate, a possible intermediate in the assimilation of CO2 and acetate journal February 1989
C1 metabolism inParacoccus denitrificans: Genetics ofParacoccus denitrificans journal April 1991
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant journal September 1992
Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-1 journal February 2007
Prokaryotic Genome Size and SSU rDNA Copy Number: Estimation of Microbial Relative Abundance from a Mixed Population journal August 1999
Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase journal May 2007
Genomic organization and biochemistry of the ribulose monophosphate pathway and its application in biotechnology journal July 2009
Developments and perspectives of photobioreactors for biofuel production journal June 2010
Assessment of heterologous butyrate and butanol pathway activity by measurement of intracellular pathway intermediates in recombinant Escherichia coli journal July 2010
Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera journal July 2010
Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator N-1 journal February 2016
Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus journal November 2009
Discoveries in Rubisco (Ribulose 1,5-bisphosphate carboxylase/oxygenase): a historical perspective journal July 2007
Rapid monitoring of transesterification and assessing biodiesel fuel quality by near-infrared spectroscopy using a fiber-optic probe journal July 1999
Developments in Directed Evolution for Improving Enzyme Functions journal August 2007
Alkane biosynthesis by decarbonylation of aldehyde catalyzed by a microsomal preparation from Botryococcus braunii journal June 1991
A general method applicable to the search for similarities in the amino acid sequence of two proteins journal March 1970
Identification of common molecular subsequences journal March 1981
Evolutionary changes in the genetic code journal November 1993
Production of ubiquinone in Escherichia coli by expression of various genes responsible for ubiquinone biosynthesis journal January 1995
Chapter thirteen - Assembly of BioBrick Standard Biological Parts Using Three Antibiotic Assembly book January 2011
C4-dicarboxylate carriers and sensors in bacteria journal January 2002
Carbon isotopic fractionation associated with lipid biosynthesis by a cyanobacterium: Relevance for interpretation of biomarker records journal December 1997
Controls on the stable isotope composition of seasonal growth bands in aragonitic fresh-water bivalves (unionidae) journal April 1999
Thermal regulation of membrane fluidity in Escherichia coli. Effects of overproduction of beta-ketoacyl-acyl carrier protein synthase I. journal February 1983
Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase. journal October 1990
Purification and Properties of α-Ketoglutarate Synthase from a Photosynthetic Bacterium journal November 1972
Biosynthetic control of the natural abundance of carbon 13 at specific positions within fatty acids in Escherichia coli. Evidence regarding the coupling of fatty acid and phospholipid synthesis. journal December 1980
Basic local alignment search tool journal October 1990
[20] Fusions to maltose-binding protein: Control of folding and solubility in protein purification book January 2000
Experimental Evolution in Yeast book January 2010
Knowledge discovery in gene-expression-microarray data: mining the information output of the genome journal November 1999
Gene-expression tools for the metabolic engineering of bacteria journal November 1999
Towards novel processes for the fine-chemical and pharmaceutical industries journal August 2002
DNA chips: promising toys have become powerful tools journal May 1999
Crystal Structure of MJ1247 Protein from M. jannaschii at 2.0 Å Resolution Infers a Molecular Function of 3-Hexulose-6-Phosphate Isomerase journal February 2002
Protein evolution by molecular breeding journal June 1999
A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production journal March 2010
Ethanol fermentation technologies from sugar and starch feedstocks journal January 2008
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal August 2005
Microbiology of synthesis gas fermentation for biofuel production journal June 2007
Limitations and prospects of natural photosynthesis for bioenergy production journal June 2010
A novel NADP+-dependent formate dehydrogenase from Burkholderia stabilis 15516: Screening, purification and characterization journal June 2010
Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters journal June 2005
Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains journal February 2012
Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli journal September 2011
Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability journal July 2016
Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria journal February 2009
Circular permutation: a different way to engineer enzyme structure and function journal January 2011
Ensemble modeling for strain development of l-lysine-producing Escherichia coli journal July 2009
Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase journal July 2011
Engineering Escherichia coli for methanol conversion journal March 2015
Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli journal January 2017
Prediction of protein function from protein sequence and structure journal January 1999
A Novel Δ32-Enoyl-CoA Isomerase Involved in the Biosynthesis of the Cyclohexanecarboxylic Acid-Derived Moiety of the Polyketide Ansatrienin A journal June 2000
Biodiesel: The Use of Vegetable Oils and Their Derivatives as Alternative Diesel Fuels book May 1997
Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview journal October 2007
The Path of Carbon in Photosynthesis. XXI. The Cyclic Regeneration of Carbon Dioxide Acceptor 1 journal April 1954
Synthesis of Methyl Halides from Biomass Using Engineered Microbes journal May 2009
A single mutation in the NAD-specific formate dehydrogenase from Candida methylica allows the enzyme to use NADP journal January 2001
Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin journal September 2000
Directed evolution of proteins by exon shuffling journal May 2001
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega journal January 2011
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection journal February 2006
Environmental signal integration by a modular AND gate journal August 2007
Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals journal August 2011
Refinement and standardization of synthetic biological parts and devices journal July 2008
Metabolic gene–deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes journal September 2004
Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency journal June 2007
The hexose phosphate synthetase of Methylococcus capsulatus journal April 1972
Hexose phosphate synthetase from Methylococcus capsulatus makes d–arabino-3-hexulose phosphate journal April 1974
The carbon assimilation pathways of Methylococcus capsulatus, Pseudomonas methanica and Methylosinus trichosporium (OB3B) during growth on methane journal December 1974
Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus journal December 1974
Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae journal November 2002
[13C]propionate oxidation in wild-type and citrate synthase mutant Escherichia coli: evidence for multiple pathways of propionate utilization journal May 1993
Mechanistic diversity and regulation of Type II fatty acid synthesis journal November 2002
A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6 journal December 2003
Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola: A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle journal April 2002
Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase journal June 2002
Biosynthesis of ansatrienin (mycotrienin) and naphthomycin. Identification and analysis of two separate biosynthetic gene clusters in Streptomyces collinus Tu 1892 journal March 1999
Design and analysis of synthetic carbon fixation pathways journal April 2010
Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus journal December 2009
Scaffoldless engineered enzyme assembly for enhanced methanol utilization journal October 2016
Carbon Isotope Fractionation In Formation Of Amino Acids By Photosynthetic Organisms journal May 1961
A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium. journal April 1966
Alkane biosynthesis by decarbonylation of aldehydes catalyzed by a particulate preparation from Pisum sativum. journal November 1984
Expression and assembly of active cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli containing stoichiometric amounts of large and small subunits. journal September 1985
Rapid and efficient site-specific mutagenesis without phenotypic selection. journal January 1985
Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. journal March 1990
Applications and statistics for multiple high-scoring segments in molecular sequences. journal June 1993
Sulfide-Quinone Reductase from Rhodobacter capsulatus: Purification, Cloning, And Expression journal April 1997
Propionyl-Coenzyme A Synthase from Chloroflexus aurantiacus, a Key Enzyme of the 3-Hydroxypropionate Cycle for Autotrophic CO2 Fixation journal January 2002
A Bicyclic Autotrophic CO2 Fixation Pathway in Chloroflexus aurantiacus journal April 2002
The FabR (YijC) Transcription Factor Regulates Unsaturated Fatty Acid Biosynthesis in Escherichia coli journal February 2002
Identification of a Magnesium-dependent NAD(P)(H)-binding Domain in the Nicotinoprotein Methanol Dehydrogenase from Bacillus methanolicus journal September 2002
A New Mechanism for Anaerobic Unsaturated Fatty Acid Formation in Streptococcus pneumoniae journal September 2002
A Novel Bifunctional Wax Ester Synthase/Acyl-CoA:Diacylglycerol Acyltransferase Mediates Wax Ester and Triacylglycerol Biosynthesis in Acinetobacter calcoaceticus ADP1 journal December 2002
Enhancement and Selective Production of Phoslactomycin B, a Protein Phosphatase IIa Inhibitor, through Identification and Engineering of the Corresponding Biosynthetic Gene Cluster journal June 2003
The Soluble and Membrane-bound Transhydrogenases UdhA and PntAB Have Divergent Functions in NADPH Metabolism of Escherichia coli journal December 2003
Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester Fermentation from Euglena gracilis Defines a New Family of Enzymes Involved in Lipid Synthesis journal November 2004
InterProScan - an integration platform for the signature-recognition methods in InterPro journal September 2001
Use of the ‘Perceptron’ algorithm to distinguish translational initiation sites inE. coli journal January 1982
Compilation and analysis of Escherichia coli promoter DNA sequences journal January 1983
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal September 1997
Codon catalog usage and the genome hypothesis journal January 1980
MUSCLE: multiple sequence alignment with high accuracy and high throughput journal March 2004
KAAS: an automatic genome annotation and pathway reconstruction server journal May 2007
The National Center for Biotechnology Information's Protein Clusters Database journal October 2008
Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli journal January 2006
Expression of Bacterial Rubisco Genes in Escherichia coli
  • Gutteridge, S.; Phillips, A. L.; Kettleborough, C. A.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 313, Issue 1162, p. 433-445 https://doi.org/10.1098/rstb.1986.0050
journal October 1986
Proposal for the reclassification of Thiobacillus novellus as Starkeya novella gen. nov., comb. nov., in the alpha-subclass of the Proteobacteria. journal September 2000
Autotrophic Metabolism of Formate by Thiobacillus Strain A2 journal September 1979
Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent journal July 2002
Methylothermus subterraneus sp. nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer journal November 2011
Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents journal July 2008
Microdiesel: Escherichia coli engineered for fuel production journal September 2006
Estimation of Nitrogenase Using a Colorimetric Determination for Ethylene journal June 1973
Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays journal May 2000
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused journal January 1999
Molecular and genetical analysis of the fructose-glucose transport system in the cyanobacterium Synechocystis PCC6803 journal September 1989
A novel enzyme, citryl-CoA synthetase, catalysing the first step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK-6 journal March 2004
A novel enzyme, citryl-CoA lyase, catalysing the second step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK-6 journal March 2004
GlcP constitutes the major glucose uptake system of Streptomyces coelicolor A3(2) journal December 2004
A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6 journal November 2006
Retrobiosynthetic analysis of carbon fixation in the phototrophic eubacterium Chloroflexus aurantiacus journal August 1993
Enzymes of a novel autotrophic CO2 fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle journal August 1993
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in different lineages within the phylum Aquificae: evidence for two ways of citrate cleavage journal January 2007
Quorum sensing: the many languages of bacteria journal January 2006
Regulation of quorum sensing in Pseudomonas journal March 2006
Sulfide : quinone oxidoreductase (SQR) from the lugworm Arenicola marina shows cyanide- and thioredoxin-dependent activity: Thioredoxin-dependent activity of lugworm SQR journal February 2008
Fed-Batch Culture with Automatic Feedback Control: An Advanced Operational Mode of Microbial Reaction journal May 1986
Bacterial Small-Molecule Signaling Pathways journal February 2006
A Genomic Perspective on Protein Families journal October 1997
Coassimilation of Organic Substrates via the Autotrophic 3-Hydroxypropionate Bi-Cycle in Chloroflexus aurantiacus journal July 2011
Rehosting of Bacterial Chaperones for High-Quality Protein Production journal October 2009
Metabolic Impact of Increased NADH Availability in Saccharomyces cerevisiae journal December 2009
Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism journal January 2015
Genetically Engineered SaccharomycesYeast Capable of Effective Cofermentation of Glucose and Xylose journal May 1998
New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria journal June 1998
Coordinated, Differential Expression of Two Genes through Directed mRNA Cleavage and Stabilization by Secondary Structures journal December 2000
Growth and Phylogenetic Properties of Novel Bacteria Belonging to the Epsilon Subdivision of the Proteobacteria Enriched from Alvinella pompejana and Deep-Sea Hydrothermal Vents journal October 2001
Formate as the Main Branch Point for Methylotrophic Metabolism in Methylobacterium extorquens AM1 journal May 2008
The Ribulose Monophosphate Pathway Substitutes for the Missing Pentose Phosphate Pathway in the Archaeon Thermococcus kodakaraensis journal June 2006
Malonyl-Coenzyme A Reductase in the Modified 3-Hydroxypropionate Cycle for Autotrophic Carbon Fixation in Archaeal Metallosphaera and Sulfolobus spp. journal October 2006
The L-Tartrate/Succinate Antiporter TtdT (YgjE) of L-Tartrate Fermentation in Escherichia coli journal December 2006
Properties of R-Citramalyl-Coenzyme A Lyase and Its Role in the Autotrophic 3-Hydroxypropionate Cycle of Chloroflexus aurantiacus journal January 2007
Presence of Acetyl Coenzyme A (CoA) Carboxylase and Propionyl-CoA Carboxylase in Autotrophic Crenarchaeota and Indication for Operation of a 3-Hydroxypropionate Cycle in Autotrophic Carbon Fixation † journal January 1999
A Role for the umuDC Gene Products of Escherichia coli in Increasing Resistance to DNA Damage in Stationary Phase by Inhibiting the Transition to Exponential Growth journal February 2000
A Novel Operon Encoding Formaldehyde Fixation: the Ribulose Monophosphate Pathway in the Gram-Positive Facultative Methylotrophic Bacterium Mycobacterium gastri MB19 journal February 2000
L-Malyl-Coenzyme A Lyase/β-Methylmalyl-Coenzyme A Lyase from Chloroflexus aurantiacus, a Bifunctional Enzyme Involved in Autotrophic CO2 Fixation journal November 2002
Malonyl-Coenzyme A Reductase from Chloroflexus aurantiacus, a Key Enzyme of the 3-Hydroxypropionate Cycle for Autotrophic CO2 Fixation journal May 2002
Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli journal December 2003
The Archaeon Pyrococcus horikoshii Possesses a Bifunctional Enzyme for Formaldehyde Fixation via the Ribulose Monophosphate Pathway journal May 2005
Alteration of the Fatty Acid Profile of Streptomyces coelicolor by Replacement of the Initiation Enzyme 3-Ketoacyl Acyl Carrier Protein Synthase III (FabH) journal May 2005
New Pathway for Long-Chain n-Alkane Synthesis via 1-Alcohol in Vibrio furnissii M1 journal February 2005
The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH journal April 2005
Properties of Succinyl-Coenzyme A:L-Malate Coenzyme A Transferase and Its Role in the Autotrophic 3-Hydroxypropionate Cycle of Chloroflexus aurantiacus journal March 2006
Formaldehyde incorporation by a new methylotroph (L3). journal January 1978
L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp. journal January 1990
Nalidixic Acid-Resistant Mutants of Escherichia coli Deficient in Isocitrate Dehydrogenase journal January 1971
Formate dehydrogenase of Clostridium pasteurianum. journal January 1984
A second branched-chain alpha-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins. journal June 1995
Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli. journal September 1996
A novel alternate anaplerotic pathway to the glyoxylate cycle in streptomycetes. journal August 1997
Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance journal June 1991
Sulfide oxidation in gram-negative bacteria by expression of the sulfide–quinone reductase gene of Rhodobacter capsulatus and by electron transport to ubiquinone journal September 2001
Beyond the Calvin Cycle: Autotrophic Carbon Fixation in the Ocean journal January 2011
Carbon Isotope Discrimination and Photosynthesis journal June 1989
Side effects of chaperone gene co-expression in recombinant protein production journal January 2010
Engineering BioBrick vectors from BioBrick parts journal January 2008
Measuring the activity of BioBrick promoters using an in vivo reference standard journal January 2009
Predicting specificity in bZIP coiled-coil protein interactions journal January 2004
Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris journal February 2020
Two Tandemly Arranged Ferredoxin Genes in the Hydrogenobacter thermophilus Genome: Comparative Characterization of the Recombinant [4Fe–4S] Ferredoxins journal January 2005
Adaptations to Submarine Hydrothermal Environments Exemplified by the Genome of Nautilia profundicola journal February 2009
Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production journal May 2010
Pathways of Carbon and Energy Metabolism of the Epibiotic Community Associated with the Deep-Sea Hydrothermal Vent Shrimp Rimicaris exoculata journal January 2011
Metabolic Deficiences Revealed in the Biotechnologically Important Model Bacterium Escherichia coli BL21(DE3) journal August 2011
Methylotrophic Bacillus methanolicus Encodes Two Chromosomal and One Plasmid Born NAD+ Dependent Methanol Dehydrogenase Paralogs with Different Catalytic and Biochemical Properties journal March 2013
GeneAssist book January 1997
Genome-Scale Thermodynamic Analysis of Escherichia coli Metabolism journal February 2006
Idempotent Vector Design for Standard Assembly of Biobricks report January 2003
Fractionation of Carbon and Hydrogen Isotopes in Biosynthetic Processes journal January 2001
Bioengineering for Microbial Inulinases: Trends and Applications journal July 2017
Prerequisites for amplicon pyrosequencing of microbial methanol utilizers in the environment journal January 2013
Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters journal May 1969
Comparison of methods to determine algal δ 13 C in freshwater: Algal δ 13 C in freshwater journal January 2008

Similar Records

Methods for making chemoautotrophic cells by engineering an energy conversion pathway and a carbon fixation pathway
Patent · Tue Oct 13 00:00:00 EDT 2020 · OSTI ID:1771496

Methods and systems for chemoautotrophic production of organic compounds
Patent · Mon Feb 26 23:00:00 EST 2018 · OSTI ID:1423445

Methods and systems for chemoautotrophic production of organic compounds
Patent · Mon Jan 07 23:00:00 EST 2013 · OSTI ID:1082897

Related Subjects