Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure

Journal Article · Wed Aug 01 04:00:00 EDT 2012 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/J.TAAP.2012.05.013· OSTI ID:22215872

Huang, Chao-Yuan ^[1]; Su, Chien-Tien ^[2]; Chung, Chi-Jung ^[3]; Pu, Yeong-Shiau ^[4]; Chu, Jan-Show ^[1]; Yang, Hsiu-Yuan ^[5]; Wu, Chia-Chang ^[5]; Hsueh, Yu-Mei ^[6]

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1. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan (China)
2. Department of Family Medicine, Taipei Medical University Hospital, Taipei, Taiwan (China)
3. Department of Health Risk Management, College of Public Health, China Medical University, Taichung, Taiwan (China)
4. Department of Urology, National Taiwan University Hospital, College of Medicine National Taiwan University, Taipei, Taiwan (China)
5. School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China)
6. Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan (China)

8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case–control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC–MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose–response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p = 0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC. -- Highlights: ► Urinary 8-OHdG was significantly related to urinary total arsenic. ► Higher urinary 8-OHdG was a strong predictor of RCC risk. ► Urinary 8-OHdG may modify arsenic related RCC risk.

• OSTI ID: 22215872
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 3 Vol. 262; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABSORPTION SPECTROSCOPY
ARSENIC
BIOPSY
CARCINOMAS
CONCENTRATION RATIO
HEALTH HAZARDS
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
HOSPITALS
IMAGES
KIDNEYS
MASS SPECTROSCOPY
MULTIVARIATE ANALYSIS
PATIENTS