DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication

Journal Article · Fri Apr 16 04:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.03.086· OSTI ID:22202476

Cha, Seho ^[1]; Lim, Chunghun ^[2]; Lee, Jae Young ^[1]; Song, Yoon-Jae ^[3]; Park, Junsoo ^[4]; Choe, Joonho ^[2]; Seo, Taegun ^[1]

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1. Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of)
2. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Korea, Republic of)
3. Department of Life Science, Kyungwon University, Seongnam-Si, Kyeonggi-Do 461-701 (Korea, Republic of)
4. Division of Biological Science and Technology, Yonsei University, Wonju 220-100 (Korea, Republic of)

During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.

• OSTI ID: 22202476
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 394; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIGENS
DNA
HERPES SIMPLEX
PROTEINS
SARCOMAS
VIRAL DISEASES