Protein complexes associated with the Kaposi's sarcoma-associated herpesvirus-encoded LANA

Journal Article · Wed Aug 01 04:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2007.03.010· OSTI ID:20977045

Kaul, Rajeev ^[1]; Verma, Subhash C ^[1]; Robertson, Erle S ^[1]

---

1. Department of Microbiology and Tumor Virology Program of the Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, 201E Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 (United States)

Kaposi's sarcoma-associated herpesvirus (KSHV) is the major biological cofactor contributing to development of Kaposi's sarcoma. KSHV establishes a latent infection in human B cells expressing the latency-associated nuclear antigen (LANA), a critical factor in the regulation of viral latency. LANA is known to modulate viral and cellular gene expression. We report here on some initial proteomic studies to identify cellular proteins associated with the amino and carboxy-terminal domains of LANA. The results of these studies show an association of known cellular proteins which support LANA functions and have identified additional LANA-associated proteins. These results provide new evidence for complexes involving LANA with a number of previously unreported functional classes of proteins including DNA polymerase, RNA helicase and cell cycle control proteins. The results also indicate that the amino terminus of LANA can interact with its carboxy-terminal domain. This interaction is potentially important for facilitating associations with other cell cycle regulatory proteins which include CENP-F identified in association with both the amino and carboxy-termini. These novel associations add to the diversity of LANA functions in relation to the maintenance of latency and subsequent transformation of KSHV infected cells.

• OSTI ID: 20977045
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 364; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIGENS
CELL CYCLE
DNA POLYMERASES
GENE REGULATION
GENES
RNA
SARCOMAS