Bacteroides fragilis induce necrosis on mice peritoneal macrophages: In vitro and in vivo assays

Journal Article · Fri Oct 02 04:00:00 EDT 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.05.124· OSTI ID:22199811

Vieira, J. M.B.D., ^[1]; Seabra, S. H. ^[1]; Vallim, D. C. ^[2]; Americo, M. A.; Fracallanza, S. E.L. ^[3]; Vommaro, R. C. ^[4]; Domingues, R. M.C.P. ^[5]

---

1. Laboratorio de Tecnologia em Cultura de Celulas, UEZO, Rio de Janeiro (Brazil)
2. Instituto Oswaldo Cruz, Rio de Janeiro (Brazil)
3. Laboratorio de Bacteriologia Medica, IMPPG, UFRJ, Rio de Janeiro (Brazil)
4. Laboratorio de Ultra-estrutura Celular Hertha Meyer, IBCCF, UFRJ (Brazil)
5. Laboratorio de Biologia de Anaerobios, IMPPG, UFRJ, Rio de Janeiro (Brazil)

Bacteroides fragilis is an anaerobic bacteria component of human intestinal microbiota and agent of infections. In the host B. fragilis interacts with macrophages, which produces toxic radicals like NO. The interaction of activated mice peritoneal macrophages with four strains of B. fragilis was evaluated on this study. Previously was shown that such strains could cause metabolic and morphologic alterations related to macrophage death. In this work propidium iodide staining showed the strains inducing macrophage necrosis in that the labeling was evident. Besides nitroblue tetrazolium test showed that B. fragilis stimulates macrophage to produce oxygen radicals. In vivo assays performed in BalbC mice have results similar to those for in vitro tests as well as scanning electron microscopy, which showed the same surface pore-like structures observed in vitro before. The results revealed that B. fragilis strains studied lead to macrophage death by a process similar to necrosis.

• OSTI ID: 22199811
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 387; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BACTERIA
IN VITRO
IN VIVO
MACROPHAGES
MICE
NECROSIS
NITRIC OXIDE
SCANNING ELECTRON MICROSCOPY
TETRAZOLIUM
TOXICITY