Dielectric barrier discharge plasma in Ar/O{sub 2} promoting apoptosis behavior in A549 cancer cells

Journal Article · Mon Dec 19 00:00:00 EST 2011 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.3666819· OSTI ID:22027851

Jun, Huang; Hui, Li; Wei, Chen; Guohua, Lv; Xingquan, Wang; Guoping, Zhang; Pengye, Wang ^[1]; Ostrikov, Kostya ^[2]; Size, Yang ^[1]

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1. Institute of Physics, Chinese Academy of Sciences, Beijing 100190 (China)
2. Plasma Nanoscience Centre Australia (PNCA), CSIRO Materials Science and Engineering, P.O. Box 218, Lindfield, New South Wales 2070 (Australia)

The Ar/O{sub 2} plasma needle in the induction of A549 cancer cells apoptosis process is studied by means of real-time observation. The entire process of programmed cell death is observed. The typical morphological changes of A549 apoptosis are detected by 4', 6-diamidino-2-phenylindole staining, for example, chromatin condensation and nuclear fragmentation. Cell viability is determined and quantified by neutral red uptake assay, and the survival rate of A549 from Ar/O{sub 2} plasmas is presented. Further spectral analysis indicates the reactive species, including O and OH play crucial roles in the cell inactivation.

• OSTI ID: 22027851
• Journal Information: Applied Physics Letters, Vol. 99, Issue 25; Other Information: (c) 2011 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
70 PLASMA PHYSICS AND FUSION TECHNOLOGY
AMINES
APOPTOSIS
ARGON
BIOLOGICAL RADIATION EFFECTS
CHROMATIN
DIELECTRIC MATERIALS
ELECTRIC DISCHARGES
INACTIVATION
IONIZING RADIATIONS
MORPHOLOGICAL CHANGES
NEOPLASMS
NUCLEAR FRAGMENTATION
OXYGEN
PATIENTS
PLASMA
PYRAZINES
RADIOTHERAPY
UPTAKE
VIABILITY