Physical mapping of the human glutamine:fructose-6-phosphate amidotransferase gene (GFPT) to chromosome 2p13

Journal Article · Mon Mar 20 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1016/0888-7543(95)80235-E· OSTI ID:219912

Whitmore, T E; Mudri, S L; McKnight, G L ^[1]

---

1. ZymoGenetics, Inc., Seattle, WA (United States)

Diabetic hyperglycemia influences insulin resistance through a process termed glucose toxicity. Implicated as a source of the mediators of this toxicity is an increased intracellular glucose metabolism through the hexosamine pathway. The hexosamine pathway itself is controlled by the rate-limiting enzyme glutamine:fructose-6-phosphate amidotransferase (GFAT), which is the first enzyme of the pathway. It has been shown that there is a close correlation between the glucose-mediated reduction of GFAT activity and the onset of insulin desensitization of the glucose transport system, a condition associated with insulin-resistant states of non-insulin-dependent diabetes mellitus and obesity. To gain a better understanding of the molecular regulation of GFAT and its role in the induction of insulin resistance, we previously isolated and cloned the cDNA for the human form of this enzyme and expressed the functional protein in Escherichia coli. 9 refs., 1 fig.

• OSTI ID: 219912
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 26; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DIABETES MELLITUS
DNA HYBRIDIZATION
DNA-CLONING
ENZYMES
FLUORESCENCE
GENES
GENETIC MAPPING
GLUCOSE
HUMAN CHROMOSOME 2
METABOLISM