Effects of UVA irradiation, aryl azides, and reactive oxygen species on the orthogonal inactivation of the human immunodeficiency virus (HIV-1)

Journal Article · Mon Aug 15 00:00:00 EDT 2011 · Virology

DOI:https://doi.org/10.1016/j.virol.2011.06.007· OSTI ID:21587879

Belanger, Julie M ^[1]; Raviv, Yossef; Viard, Mathias ^[2]; Cruz, M Jason de la; Nagashima, Kunio ^[3]; Blumenthal, Robert ^[1]

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1. Center for Cancer Research Nanobiology Program, National Cancer Institute Frederick (United States)
2. Basic Research Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702 (United States)
3. Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702 (United States)

Previously we reported that hydrophobic aryl azides partition into hydrophobic regions of the viral membrane of enveloped viruses and inactivate the virus upon UVA irradiation for 2 min. Prolonged irradiation (15 min) resulted in viral protein aggregation as visualized via Western blot analysis, due to reactive oxygen species (ROS) formation, with preservation of the surface antigenic epitopes. Herein, we demonstrate that these aggregates show detergent resistance and that this property may be useful towards the creation of a novel orthogonal virus inactivation strategy for use in preparing experimental vaccines. When ROS-modified HIV virus preparations were treated with 1% Triton X-100, there was an increase in the percent of viral proteins (gp41, p24) in the viral pellet after ultracentrifugation through sucrose. Transmission electron microscopy (TEM) of these detergent-resistant pellets shows some recognizable virus fragments, and immunoprecipitation studies of the gp41 aggregates suggest the aggregation is covalent in nature, involving short-range interactions.

• OSTI ID: 21587879
• Journal Information: Virology, Vol. 417, Issue 1; Other Information: DOI: 10.1016/j.virol.2011.06.007; PII: S0042-6822(11)00271-6; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AIDS VIRUS
AZIDES
DETERGENTS
INACTIVATION
INTERACTION RANGE
IRRADIATION
MEMBRANES
OXYGEN
PELLETS
PRESERVATION
PROTEINS
SACCHAROSE
TRANSMISSION ELECTRON MICROSCOPY
TRITONS
ULTRACENTRIFUGATION
VACCINES
ADDITIVES
CARBOHYDRATES
CENTRIFUGATION
CHARGED PARTICLES
DISACCHARIDES
DISTANCE
ELECTRON MICROSCOPY
ELEMENTS
EMULSIFIERS
MICROORGANISMS
MICROSCOPY
NITROGEN COMPOUNDS
NONMETALS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
SACCHARIDES
SEPARATION PROCESSES
SURFACTANTS
VIRUSES
WETTING AGENTS