Complete inactivation of Venezuelan equine encephalitis virus by 1,5-iodonaphthylazide

Journal Article · Fri Jun 29 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.04.115· OSTI ID:20991403

Sharma, Anuj ^[1]; Raviv, Yossef ^[2]; Puri, Anu ^[3]; Viard, Mathias ^[2]; Blumenthal, Robert ^[3]; Maheshwari, Radha K ^[1]

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1. Centre for Combat Casualty and Life Sustainment Research, Department of Pathology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 (United States)
2. CCR Nanobiology Program, Centre for Cancer Research, National Cancer Research Institute, Basic Research Program SAIC Frederick, Frederick, MD (United States)
3. CCR Nanobiology Program, Centre for Cancer Research, National Cancer Research Institute, Frederick, MD (United States)

Hydrophobic alkylating compounds like 1,5-iodonaphthylazide (INA) partitions into biological membranes and accumulates selectively into the hydrophobic domain of the lipid bilayer. Upon irradiation with far UV light, INA binds selectively to transmembrane proteins in the viral envelope and renders them inactive. Such inactivation does not alter the ectodomains of the membrane proteins thus preserving the structural and conformational integrity of immunogens on the surface of the virus. In this study, we have used INA to inactivate Venezuelan equine encephalitis virus (VEEV). Treatment of VEEV with INA followed by irradiation with UV light resulted in complete inactivation of the virus. Immuno-fluorescence for VEEV and virus titration showed no virus replication in-vitro. Complete loss of infectivity was also achieved in mice infected with INA treated plus irradiated preparations of VEEV. No change in the structural integrity of VEEV particles were observed after treatment with INA plus irradiation as assessed by electron microscopy. This data suggest that such inactivation strategies can be used for developing vaccine candidates for VEEV and other enveloped viruses.

• OSTI ID: 20991403
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 358; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AZIDES
ELECTRON MICROSCOPY
ENCEPHALITIS
FLUORESCENCE
IN VITRO
INFECTIVITY
IRRADIATION
LIPIDS
MEMBRANE PROTEINS
MICE
TITRATION
ULTRAVIOLET RADIATION
VIRUSES