Low-frequency four-wave mixing spectroscopy of biomolecules in aqueous solutions
- Wave Research Center, A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)
Four-wave mixing (FWM) spectroscopy is used to detect the rotational resonances of H{sub 2}O and H{sub 2}O{sub 2} molecules in DNA and denatured DNA aqueous solutions in the range {+-}10 cm{sup -1} with a spectral resolution of 3 GHz. It is found that the resonance contribution of the rotational transitions of these molecules increases significantly in solutions rather than in distilled water. This fact is interpreted as a manifestation of specific properties of a hydration layer at DNA-water and denatured DNA-water interfaces. Analysis of the FWM spectra shows that the concentration of H{sub 2}O{sub 2} molecules in the hydration layer of the DNA solution increases by a factor of 3 after denaturation. The FWM spectra of aqueous solutions of {alpha}-chymotrypsin protein are obtained in the range {+-}7cm{sup -1} at the protein concentrations between 0 and 20 mg cm{sup -3}. It is found that the hypersound velocity in the protein aqueous solution, measured by the shift of Brillouin components in the scattering spectrum, obeys a cubic dependence on the protein concentration and reaches a value of about 3000 m s{sup -1} at 20 mg cm{sup -3}. (application of lasers and laser-optical methods in life sciences)
- OSTI ID:
- 21541814
- Journal Information:
- Quantum Electronics (Woodbury, N.Y.), Vol. 40, Issue 12; Other Information: DOI: 10.1070/QE2010v040n12ABEH014471; ISSN 1063-7818
- Country of Publication:
- United States
- Language:
- English
Similar Records
Four-photon Rayleigh-wing spectroscopy of the aqueous solution of {alpha}-chymotrypsin protein
Four-photon microwave laser spectroscopy of molecules in the hydration layers of biopolymers and nanoparticles
Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
AQUEOUS SOLUTIONS
CHYMOTRYPSIN
DNA
FREQUENCY MIXING
GHZ RANGE 01-100
HYDRATION
HYDROGEN PEROXIDE
LASERS
SCATTERING
SPECTRA
SPECTROSCOPY
WATER
DISPERSIONS
ENZYMES
FREQUENCY RANGE
GHZ RANGE
HOMOGENEOUS MIXTURES
HYDROGEN COMPOUNDS
HYDROLASES
MIXTURES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEROXIDES
PROTEINS
SERINE PROTEINASES
SOLUTIONS
SOLVATION