Four-photon Rayleigh-wing spectroscopy of the aqueous solution of {alpha}-chymotrypsin protein

Journal Article · Mon Jul 31 00:00:00 EDT 2006 · Quantum Electronics (Woodbury, N.Y.)

DOI:https://doi.org/10.1070/QE2006V036N07ABEH013182· OSTI ID:21466557

Bunkin, Aleksei F; Nurmatov, Alisher A; Pershin, Sergei M ^[1]

---

1. A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)

The spectra of coherent librations of H{sub 2}O molecules, coinciding in frequencies with the rotational spectrum of gaseous H{sub 2}O, are observed for the first time in aqueous solutions of {alpha}-chymotrypsin protein and hydrogen peroxide and in deionised Milli-Q water by the method of four-photon laser scattering in the frequency range 0-100 cm{sup -1}. The resonance contribution of molecular librations to the four-photon scattering signal considerably increases in aqueous solutions compared to water. The resonances related to the lines of the ortho- and para-modifications of the natural isotope of the H{sub 2}O molecule in the gas phase are recorded in the four-photon scattering spectra. It is found that the protein molecule in aqueous solution selectively interacts with the para-H{sub 2}O, which makes it possible to interpret some features of the interaction of microwave radiation with biological objects and to develop a new class of laser biotechnologies. (special issue devoted to the 90th anniversary of a.m. prokhorov)

• OSTI ID: 21466557
• Journal Information: Quantum Electronics (Woodbury, N.Y.), Vol. 36, Issue 7; Other Information: DOI: 10.1070/QE2006v036n07ABEH013182; ISSN 1063-7818
• Country of Publication: United States
• Language: English

Related Subjects

74 ATOMIC AND MOLECULAR PHYSICS
AQUEOUS SOLUTIONS
BIOTECHNOLOGY
CHYMOTRYPSIN
FREQUENCY RANGE
HYDROGEN PEROXIDE
LASER SPECTROSCOPY
MICROWAVE RADIATION
MOLECULES
PHOTONS
RESONANCE
SCATTERING
SPECTRA
WATER
BOSONS
DISPERSIONS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENZYMES
HOMOGENEOUS MIXTURES
HYDROGEN COMPOUNDS
HYDROLASES
MASSLESS PARTICLES
MIXTURES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEROXIDES
PROTEINS
RADIATIONS
SERINE PROTEINASES
SOLUTIONS
SPECTROSCOPY