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Direct laser immobilization of photosynthetic material on screen printed electrodes for amperometric biosensor

Journal Article · · Applied Physics Letters
DOI:https://doi.org/10.1063/1.3562297· OSTI ID:21518306
;  [1];  [2];  [3];  [2]
  1. Department of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens (Greece)
  2. Institute of Crystallography, National Research Council, Via Salaria Km 29.300, Monterotondo Scalo, 00015 Rome (Italy)
  3. Biosensor srl, Via degli Olmetti 44, Formello, 00060 Rome (Italy)
+ Show Author Affiliations

This letter demonstrates the direct laser printing of photosynthetic material onto low cost nonfunctionalized screen printed electrodes for the fabrication of photosynthesis-based amperometric biosensors. The high kinetic energy of the transferred material induces direct immobilization of the thylakoids onto the electrodes without the use of linkers. This type of immobilization is able to establish efficient electrochemical contact between proteins and electrode, stabilizing the photosynthetic biomolecule and transporting electrons to the solid state device with high efficiency. The functionality of the laser printed biosensors was evaluated by the detection of a common herbicide such as Linuron.

OSTI ID:
21518306
Journal Information:
Applied Physics Letters, Journal Name: Applied Physics Letters Journal Issue: 9 Vol. 98; ISSN APPLAB; ISSN 0003-6951
Country of Publication:
United States
Language:
English

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Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
AMPEROMETRY
BIOPHYSICS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
DETECTION
EFFICIENCY
ELECTROCHEMISTRY
ELECTRODES
ELECTROMAGNETIC RADIATION
ELECTRONS
ELEMENTARY PARTICLES
ENERGY
FERMIONS
HERBICIDES
KINETIC ENERGY
LASER RADIATION
LEPTONS
ORGANIC COMPOUNDS
PESTICIDES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYSICS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
SOLIDS
SYNTHESIS
TITRATION
VOLUMETRIC ANALYSIS