Direct laser immobilization of photosynthetic material on screen printed electrodes for amperometric biosensor

Journal Article · Mon Feb 28 00:00:00 EST 2011 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.3562297· OSTI ID:21518306

Boutopoulos, Christos; Zergioti, Ioanna ^[1]; Touloupakis, Eleftherios ^[2]; Pezzotti, Ittalo ^[3]; Giardi, Maria Teresa ^[2]

---

1. Department of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens (Greece)
2. Institute of Crystallography, National Research Council, Via Salaria Km 29.300, Monterotondo Scalo, 00015 Rome (Italy)
3. Biosensor srl, Via degli Olmetti 44, Formello, 00060 Rome (Italy)

This letter demonstrates the direct laser printing of photosynthetic material onto low cost nonfunctionalized screen printed electrodes for the fabrication of photosynthesis-based amperometric biosensors. The high kinetic energy of the transferred material induces direct immobilization of the thylakoids onto the electrodes without the use of linkers. This type of immobilization is able to establish efficient electrochemical contact between proteins and electrode, stabilizing the photosynthetic biomolecule and transporting electrons to the solid state device with high efficiency. The functionality of the laser printed biosensors was evaluated by the detection of a common herbicide such as Linuron.

• OSTI ID: 21518306
• Journal Information: Applied Physics Letters, Vol. 98, Issue 9; Other Information: DOI: 10.1063/1.3562297; (c) 2011 American Institute of Physics; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
AMPEROMETRY
BIOPHYSICS
DETECTION
EFFICIENCY
ELECTROCHEMISTRY
ELECTRODES
ELECTRONS
HERBICIDES
KINETIC ENERGY
LASER RADIATION
PHOTOSYNTHESIS
PROTEINS
SOLIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY
FERMIONS
LEPTONS
ORGANIC COMPOUNDS
PESTICIDES
PHOTOCHEMICAL REACTIONS
PHYSICS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
SYNTHESIS
TITRATION
VOLUMETRIC ANALYSIS