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Title: Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin

Abstract

Purpose: We performed a basic investigation using white rabbits of the sustained release and embolizing effects of poly L-lactic acid microspheres (PLA) to determine their usefulness for chemoembolization.Methods: Fifteen male Japanese white rabbits were used. Sustained release of an embolizing material, EPI-PLA was accomplished with l m g of PLA containing 0.03 mg of epirubicin hydrochloride (EPI). Embolization with 50 mg of PLA (total dose of EPI l.5 mg) was performed after the renal artery of the rabbits was selected (Chemo-TAE group). A group in which a bolus of 1.5 mg EPI alone was injected through the renal artery (TAI group) was established as a control group. Furthermore, a group in which embolization was performed with 50 mg of PLA alone (TAE group) was also established. These three groups, each consisting of five rabbits, were compared.Results: Blood EPI levels were serially measured. The blood EPI level in the TAI group rapidly reached a peak more than 30 min after injection, then decreased to almost zero 24 hr after injection. In the Chemo-TAE group, the blood EPI level was transiently increased 30 min after embolization, but remained low thereafter until 24 hr after embolization. EPI levels in kidney tissue isolated 24more » hr after embolization were measured. In the Chemo-TAE group, the tissue EPI level was significantly higher than that in the TAI group. When isolated kidneys were macroscopically and histologically examined, atrophy of the entire embolized kidney, as well as infarction and necrosis in the renal cortex, were observed in both the TAE group and the Chemo-TAE group. However, there were no such findings in the TAI group. The area of the infarction in the renal cortex did not significantly differ between the Chemo-TAE group and the TAE group; however, there was vascular injury in the Chemo-TAE group and none in the TAE group.Conclusion: It was demonstrated that EPI-PLA, a chemo-embolizing material, maintained high local concentrations of the anticancer drug, while maintaining low blood levels of the anticancer drug.« less

Authors:
 [1];  [2]; ;  [1]; ;  [3];  [4]
  1. Kyoto University Hospital, Department of Radiology (Japan)
  2. Kyoto City Hospital, Department of Radiology (Japan)
  3. Kyoto University, Department of Artificial Organs, Division of Physiological Artificial Organs, Research Center for Biomedical Engineering (Japan)
  4. Kyoto University, Department of Biomaterials, Division of Molecular Design of Biomaterials, Research Center for Biomedical Engineering (Japan)
Publication Date:
OSTI Identifier:
21414359
Resource Type:
Journal Article
Journal Name:
Cardiovascular and Interventional Radiology
Additional Journal Information:
Journal Volume: 23; Journal Issue: 3; Other Information: DOI: 10.1007/s002700010046; Copyright (c) 2000 Springer-Verlag; Journal ID: ISSN 0174-1551
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTINEOPLASTIC DRUGS; ARTERIES; BLOOD; EMBOLI; INJECTION; KIDNEYS; LACTIC ACID; MICROSPHERES; RABBITS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD VESSELS; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; DRUGS; HYDROXY ACIDS; INTAKE; MAMMALS; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; VERTEBRATES

Citation Formats

Fujiwara, Kazuhisa, Hayakawa, Katsumi, Nagata, Yasushi, Hiraoka, Masahiro, Nakamura, Tatsuo, Shimizu, Yoshihiko, and Ikada, Yoshito. Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin. United States: N. p., 2000. Web. doi:10.1007/S002700010046.
Fujiwara, Kazuhisa, Hayakawa, Katsumi, Nagata, Yasushi, Hiraoka, Masahiro, Nakamura, Tatsuo, Shimizu, Yoshihiko, & Ikada, Yoshito. Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin. United States. doi:10.1007/S002700010046.
Fujiwara, Kazuhisa, Hayakawa, Katsumi, Nagata, Yasushi, Hiraoka, Masahiro, Nakamura, Tatsuo, Shimizu, Yoshihiko, and Ikada, Yoshito. Mon . "Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin". United States. doi:10.1007/S002700010046.
@article{osti_21414359,
title = {Experimental embolization of rabbit renal arteries to compare the effects of poly L-lactic acid microspheres with and without epirubicin release against ntraarterial injection of epirubicin},
author = {Fujiwara, Kazuhisa and Hayakawa, Katsumi and Nagata, Yasushi and Hiraoka, Masahiro and Nakamura, Tatsuo and Shimizu, Yoshihiko and Ikada, Yoshito},
abstractNote = {Purpose: We performed a basic investigation using white rabbits of the sustained release and embolizing effects of poly L-lactic acid microspheres (PLA) to determine their usefulness for chemoembolization.Methods: Fifteen male Japanese white rabbits were used. Sustained release of an embolizing material, EPI-PLA was accomplished with l m g of PLA containing 0.03 mg of epirubicin hydrochloride (EPI). Embolization with 50 mg of PLA (total dose of EPI l.5 mg) was performed after the renal artery of the rabbits was selected (Chemo-TAE group). A group in which a bolus of 1.5 mg EPI alone was injected through the renal artery (TAI group) was established as a control group. Furthermore, a group in which embolization was performed with 50 mg of PLA alone (TAE group) was also established. These three groups, each consisting of five rabbits, were compared.Results: Blood EPI levels were serially measured. The blood EPI level in the TAI group rapidly reached a peak more than 30 min after injection, then decreased to almost zero 24 hr after injection. In the Chemo-TAE group, the blood EPI level was transiently increased 30 min after embolization, but remained low thereafter until 24 hr after embolization. EPI levels in kidney tissue isolated 24 hr after embolization were measured. In the Chemo-TAE group, the tissue EPI level was significantly higher than that in the TAI group. When isolated kidneys were macroscopically and histologically examined, atrophy of the entire embolized kidney, as well as infarction and necrosis in the renal cortex, were observed in both the TAE group and the Chemo-TAE group. However, there were no such findings in the TAI group. The area of the infarction in the renal cortex did not significantly differ between the Chemo-TAE group and the TAE group; however, there was vascular injury in the Chemo-TAE group and none in the TAE group.Conclusion: It was demonstrated that EPI-PLA, a chemo-embolizing material, maintained high local concentrations of the anticancer drug, while maintaining low blood levels of the anticancer drug.},
doi = {10.1007/S002700010046},
journal = {Cardiovascular and Interventional Radiology},
issn = {0174-1551},
number = 3,
volume = 23,
place = {United States},
year = {2000},
month = {5}
}