Imaging Primary Lung Cancers in Mice to Study Radiation Biology

Journal Article · Mon Mar 15 00:00:00 EDT 2010 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2009.11.038· OSTI ID:21372148

Grimm, Jan ^[1]; Guimaraes, Alexander R ^[1]; Wojtkiewicz, Gregory R ^[1]; Perez, Bradford A ^[2]; Santiago, Philip M ^[3]; Anthony, Nikolas K; Forbes, Thomas; Doppke, Karen ^[4]

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1. Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA (United States)
2. Departments of Radiation Oncology and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC (United States)
3. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA (United States)
4. Department of Radiation Oncology, Massachusetts General Hospital, Boston, MA (United States)

Purpose: To image a genetically engineered mouse model of non-small-cell lung cancer with micro-computed tomography (micro-CT) to measure tumor response to radiation therapy. Methods and Materials: The Cre-loxP system was used to generate primary lung cancers in mice with mutation in K-ras alone or in combination with p53 mutation. Mice were serially imaged by micro-CT, and tumor volumes were determined. A comparison of tumor volume by micro-CT and tumor histology was performed. Tumor response to radiation therapy (15.5 Gy) was assessed with micro-CT. Results: The tumor volume measured with free-breathing micro-CT scans was greater than the volume calculated by histology. Nevertheless, this imaging approach demonstrated that lung cancers with mutant p53 grew more rapidly than lung tumors with wild-type p53 and also showed that radiation therapy increased the doubling time of p53 mutant lung cancers fivefold. Conclusions: Micro-CT is an effective tool to noninvasively measure the growth of primary lung cancers in genetically engineered mice and assess tumor response to radiation therapy. This imaging approach will be useful to study the radiation biology of lung cancer.

• OSTI ID: 21372148
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 76, Issue 4; Other Information: DOI: 10.1016/j.ijrobp.2009.11.038; PII: S0360-3016(09)03604-9; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CAT SCANNING
GROWTH
HISTOLOGY
IMAGES
LUNGS
MICE
MUTANTS
NEOPLASMS
RADIOBIOLOGY
RADIOTHERAPY
ANIMALS
BIOLOGY
BODY
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
DISEASES
MAMMALS
MEDICINE
NUCLEAR MEDICINE
ORGANS
RADIOLOGY
RESPIRATORY SYSTEM
RODENTS
THERAPY
TOMOGRAPHY
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