Uniform silica nanoparticles encapsulating two-photon absorbing fluorescent dye

Journal Article · Wed Apr 15 00:00:00 EDT 2009 · Journal of Solid State Chemistry

OSTI ID:21370349

Weibing, Wu ^[1]; Chang, Liu ^[2]; Wei, Huang ^[2]; Shengrui, Zhou; Wei, Jiang; Yueming, Sun ^[1]; Yiping, Cui; Chunxinag, Xu ^[2]

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1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189 (China)
2. Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096 (China)

We have prepared uniform silica nanoparticles (NPs) doped with a two-photon absorbing zwitterionic hemicyanine dye by reverse microemulsion method. Obvious solvatochromism on the absorption spectra of dye-doped NPs indicates that solvents can partly penetrate into the silica matrix and then affect the ground and excited state of dye molecules. For dye-doped NP suspensions, both one-photon and two-photon excited fluorescence are much stronger and recorded at shorter wavelength compared to those of free dye solutions with comparative overall dye concentration. This behavior is possibly attributed to the restricted twisted intramolecular charge transfer (TICT), which reduces fluorescence quenching when dye molecules are trapped in the silica matrix. Images from two-photon laser scanning fluorescence microscopy demonstrate that the dye-doped silica NPs can be actively uptaken by Hela cells with low cytotoxicity. - Graphical abstract: Water-soluble silica NPs doped with a two-photon absorbing zwitterionic hemicyanine dye were prepared. They were found of enhanced one-photon and two-photon excited fluorescence compared to free dye solutions. Images from two-photon laser scanning fluorescence microscopy demonstrate that the dye-doped silica NPs can be actively uptaken by Hela cells.

• OSTI ID: 21370349
• Journal Information: Journal of Solid State Chemistry, Vol. 182, Issue 4; Other Information: DOI: 10.1016/j.jssc.2008.12.003; PII: S0022-4596(08)00636-1; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0022-4596
• Country of Publication: United States
• Language: English

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
DOPED MATERIALS
DYES
FLUORESCENCE
HELA CELLS
MICROEMULSIONS
NANOSTRUCTURES
PHOTONS
SILICA
ZWITTERIONIC COMPOUNDS
ANIMAL CELLS
BOSONS
COLLOIDS
DISPERSIONS
ELEMENTARY PARTICLES
EMISSION
EMULSIONS
LUMINESCENCE
MASSLESS PARTICLES
MATERIALS
MINERALS
OXIDE MINERALS
PHOTON EMISSION
POLAR COMPOUNDS
TUMOR CELLS