INOSITOL HEXAKISPHOSPHATE MEDIATES APOPTOSIS IN HUMAN BREAST ADENOCARCINOMA MCF-7 CELL LINE VIA INTRINSIC PATHWAY

Journal Article · Mon Apr 12 04:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3419682· OSTI ID:21367153

Agarwal, Rakhee ^[1]; Ali, Nawab ^[2]

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1. Department of Toxicology and Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States)
2. Graduate Institute of Technology, University of Arkansas at Little Rock, Little Rock, Arkansas (United States)

Inositol polyphosphates (InsP{sub s}) are naturally occurring compounds ubiquitously present in plants and animals. Inositol hexakisphosphate (InsP{sub 6}) is the most abundant among all InsP{sub s} and constitutes the major portion of dietary fiber in most cereals, legumes and nuts. Certain derivatives of InsP{sub s} also regulate cellular signaling mechanisms. InsP{sub s} have also been shown to reduce tumor formation and induce apoptosis in cancerous cells. Therefore, in this study, the effects of InsPs on apoptosis were studied in an attempt to investigate their potential anti-cancer therapeutic application and understand their mechanism of action. Acridine orange and ethidium bromide staining suggested that InsP{sub 6} dose dependently induced apoptosis in human breast adenocarcinoma MCF-7 cells. Among InsP{sub s} tested (InsP{sub 3}, InsP{sub 4}, InsP{sub 5}, and InsP{sub 6}), InsP{sub 6} was found to be the most effective in inducing apoptosis. Furthermore, effects of InsP{sub 6} were found most potent inducing apoptosis. Etoposide, the drug known to induce apoptosis in both in vivo and in vitro, was used as a positive control. Western blotting experiments using specific antibodies against known apoptotic markers suggested that InsP{sub 6} induced apoptotic changes were mediated via an intrinsic apoptotic pathway.

• OSTI ID: 21367153
• Journal Information: AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1229; ISSN APCPCS; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACRIDINE ORANGE
ACRIDINES
AMINES
ANIMALS
ANTIBODIES
APOPTOSIS
AROMATICS
AZAARENES
AZINES
BODY
CARBOHYDRATES
CARCINOMAS
CEREALS
DISEASES
DNA
DRUGS
DYES
GLANDS
GRAMINEAE
HETEROCYCLIC COMPOUNDS
INOSITOL
INOSITOLS
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MAMMARY GLANDS
MONOSACCHARIDES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANTS
PYRIDINES
SACCHARIDES