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Title: GABAergic influences on ORX receptor-dependent abnormal motor behaviors and neurodegenerative events in fish

Abstract

At date the major neuroreceptors i.e. gamma-aminobutyric acid{sub A} (GABA{sub A}R) and orexin (ORXR) systems are beginning to be linked to homeostasis, neuroendocrine and emotional states. In this study, intraperitoneal treatment of the marine teleost Thalassoma pavo with the highly selective GABA{sub A}R agonist (muscimol, MUS; 0,1 mug/g body weight) and/or its antagonist bicuculline (BIC; 1 mug/g body weight) have corroborated a GABA{sub A}ergic role on motor behaviors. In particular, MUS induced moderate (p < 0.05) and great (p < 0.01) increases of swimming towards food sources and resting states after 24 (1 dose) and 96 (4 doses) h treatment sessions, respectively, when compared to controls. Conversely, BIC caused a very strong (p < 0.001) reduction of the former behavior and in some cases convulsive swimming. From the correlation of BIC-dependent behavioral changes to neuronal morphological and ORXR transcriptional variations, it appeared that the disinhibitory action of GABA{sub A}R was very likely responsible for very strong and strong ORXR mRNA reductions in cerebellum valvula and torus longitudinalis, respectively. Moreover these effects were linked to evident ultra-structural changes such as shrunken cell membranes and loss of cytoplasmic architecture. In contrast, MUS supplied a very low, if any, argyrophilic reaction in hypothalamicmore » and mesencephalic regions plus a scarce level of ultra-structural damages. Interestingly, combined administrations of MUS + BIC were not related to consistent damages, aside mild neuronal alterations in motor-related areas such as optic tectum. Overall it is tempting to suggest, for the first time, a neuroprotective role of GABA{sub A}R inhibitory actions against the overexcitatory ORXR-dependent neurodegeneration and consequently abnormal swimming events in fish.« less

Authors:
 [1]; ; ;  [1]
  1. Comparative Neuroanatomy Laboratory, Ecology Department, University of Calabria, 87030 Arcavacata di Rende (Italy)
Publication Date:
OSTI Identifier:
21344868
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 243; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2009.11.015; PII: S0041-008X(09)00481-5; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINOBUTYRIC ACID; CELL MEMBRANES; CEREBELLUM; DOSES; HOMEOSTASIS; MESSENGER-RNA; RECEPTORS; SILVER; TRANSMISSION ELECTRON MICROSCOPY; AMINO ACIDS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; BRAIN; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; DRUGS; ELECTRON MICROSCOPY; ELEMENTS; MEMBRANE PROTEINS; MEMBRANES; METALS; MICROSCOPY; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RNA; TRANSITION ELEMENTS

Citation Formats

Facciolo, Rosa Maria, E-mail: rm.facciolo@unical.i, Crudo, Michele, Giusi, Giuseppina, and Canonaco, Marcello. GABAergic influences on ORX receptor-dependent abnormal motor behaviors and neurodegenerative events in fish. United States: N. p., 2010. Web. doi:10.1016/j.taap.2009.11.015.
Facciolo, Rosa Maria, E-mail: rm.facciolo@unical.i, Crudo, Michele, Giusi, Giuseppina, & Canonaco, Marcello. GABAergic influences on ORX receptor-dependent abnormal motor behaviors and neurodegenerative events in fish. United States. doi:10.1016/j.taap.2009.11.015.
Facciolo, Rosa Maria, E-mail: rm.facciolo@unical.i, Crudo, Michele, Giusi, Giuseppina, and Canonaco, Marcello. Mon . "GABAergic influences on ORX receptor-dependent abnormal motor behaviors and neurodegenerative events in fish". United States. doi:10.1016/j.taap.2009.11.015.
@article{osti_21344868,
title = {GABAergic influences on ORX receptor-dependent abnormal motor behaviors and neurodegenerative events in fish},
author = {Facciolo, Rosa Maria, E-mail: rm.facciolo@unical.i and Crudo, Michele and Giusi, Giuseppina and Canonaco, Marcello},
abstractNote = {At date the major neuroreceptors i.e. gamma-aminobutyric acid{sub A} (GABA{sub A}R) and orexin (ORXR) systems are beginning to be linked to homeostasis, neuroendocrine and emotional states. In this study, intraperitoneal treatment of the marine teleost Thalassoma pavo with the highly selective GABA{sub A}R agonist (muscimol, MUS; 0,1 mug/g body weight) and/or its antagonist bicuculline (BIC; 1 mug/g body weight) have corroborated a GABA{sub A}ergic role on motor behaviors. In particular, MUS induced moderate (p < 0.05) and great (p < 0.01) increases of swimming towards food sources and resting states after 24 (1 dose) and 96 (4 doses) h treatment sessions, respectively, when compared to controls. Conversely, BIC caused a very strong (p < 0.001) reduction of the former behavior and in some cases convulsive swimming. From the correlation of BIC-dependent behavioral changes to neuronal morphological and ORXR transcriptional variations, it appeared that the disinhibitory action of GABA{sub A}R was very likely responsible for very strong and strong ORXR mRNA reductions in cerebellum valvula and torus longitudinalis, respectively. Moreover these effects were linked to evident ultra-structural changes such as shrunken cell membranes and loss of cytoplasmic architecture. In contrast, MUS supplied a very low, if any, argyrophilic reaction in hypothalamic and mesencephalic regions plus a scarce level of ultra-structural damages. Interestingly, combined administrations of MUS + BIC were not related to consistent damages, aside mild neuronal alterations in motor-related areas such as optic tectum. Overall it is tempting to suggest, for the first time, a neuroprotective role of GABA{sub A}R inhibitory actions against the overexcitatory ORXR-dependent neurodegeneration and consequently abnormal swimming events in fish.},
doi = {10.1016/j.taap.2009.11.015},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 243,
place = {United States},
year = {Mon Feb 15 00:00:00 EST 2010},
month = {Mon Feb 15 00:00:00 EST 2010}
}