Effect of the atmospheric pressure nonequilibrium plasmas on the conformational changes of plasmid DNA
- Genetic Engineering International Cooperation Base of Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Ministry of Education, China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Luoyu Road 1037, Wuhan 430074 (China)
- College of Electrical and Electronics Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074 (China)
The cold atmospheric pressure plasma, which has been widely used for biomedical applications, may potentially affect the conformation of DNA. In this letter, an atmospheric pressure plasma plume is used to investigate its effects on the conformational changes of DNA of plasmid pAHC25. It is found that the plasma plume could cause plasmid DNA topology alteration, resulting in the percentage of the supercoiled plasmid DNA form decreased while that of the open circular and linearized form of plasmid DNA increased as detected by agrose gel electrophoresis. On the other hand, further investigation by using polymerase chain reaction method shows that the atmospheric pressure plasma jet treatments under proper conditions does not affect the genes of the plasmid DNA, which may have potential application in increasing the transformation frequency by genetic engineering.
- OSTI ID:
- 21294291
- Journal Information:
- Applied Physics Letters, Vol. 95, Issue 8; Other Information: DOI: 10.1063/1.3212739; (c) 2009 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
- Country of Publication:
- United States
- Language:
- English
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