Pulmonary toxicity and kinetic study of Cy5.5-conjugated superparamagnetic iron oxide nanoparticles by optical imaging

Journal Article · Sat Aug 15 04:00:00 EDT 2009 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2009.05.026· OSTI ID:21272633

Cho, Wan-Seob ^[1]; Cho, Minjung; Kim, Seoung Ryul; Choi, Mina; Lee, Jeong Yeon; Han, Beom Seok ^[1]; Park, Sue Nie ^[2]; Yu, Mi Kyung; Jon, Sangyong ^[3]; Jeong, Jayoung ^[1]

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1. Division of Toxicologic Pathology, Department of Toxicological Research, National Institute of Toxicological Research, Korea Food and Drug Administration, 231 Jinhoungno Eunpyung-ku, Seoul 122-704 (Korea, Republic of)
2. Division of Genetic Toxicology, Department of Toxicological Research, National Institute of Toxicological Research, Korea Food and Drug Administration, 231 Jinhoungno Eunpyung-ku, Seoul 122-704 (Korea, Republic of)
3. Research Center for Biomolecular Nanotechnology, Department of Life Science, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju 500-712 (Korea, Republic of)

Recent advances in the development of nanotechnology and devices now make it possible to accurately deliver drugs or genes to the lung. Magnetic nanoparticles can be used as contrast agents, thermal therapy for cancer, and be made to concentrate to target sites through an external magnetic field. However, these advantages may also become problematic when taking into account safety and toxicological factors. This study demonstrated the pulmonary toxicity and kinetic profile of anti-biofouling polymer coated, Cy5.5-conjugated thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) by optical imaging. Negatively charged, 36 nm-sized, Cy5.5-conjugated TCL-SPION was prepared for optical imaging probe. Cy5.5-conjugated TCL-SPION was intratracheally instilled into the lung by a non-surgical method. Cy5.5-conjugated TCL-SPION slightly induced pulmonary inflammation. The instilled nanoparticles were distributed mainly in the lung and excreted in the urine via glomerular filtration. Urinary excretion was peaked at 3 h after instillation. No toxicity was found under the concentration of 1.8 mg/kg and the half-lives of nanoparticles in the lung and urine were estimated to be about 14.4 {+-} 0.54 h and 24.7 {+-} 1.02 h, respectively. Although further studies are required, our results showed that Cy5.5-conjugated TCL-SPION can be a good candidate for use in pulmonary delivery vehicles and diagnostic probes.

• OSTI ID: 21272633
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 1 Vol. 239; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL FOULING
DELIVERY
DRUGS
EXCRETION
FILTRATION
HALF-LIFE
INFLAMMATION
IRON OXIDES
LUNGS
MAGNETIC FIELDS
NANOSTRUCTURES
NEOPLASMS
PARTICLES
POLYMERS
SUPERPARAMAGNETISM
SURGERY
THERAPY
TOXICITY
TRANSLOCATION
URINE