WGA-QD probe-based AFM detects WGA-binding sites on cell surface and WGA-induced rigidity alternation
- Department of Anesthesiology, First Affiliated Hospital, Jinan University, Guangzhou 510632 (China)
- Institute of Hematology, Jinan University, Guangzhou 510632 (China)
- MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510632 (China)
- Institute for Reproductive Immunology Research, Jinan University, Guangzhou 510632 (China)
- Department of Chemistry, Jinan University, Guangzhou 510632 (China)
- Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612 (United States)
A strategy involving the conjugation of fluorescent quantum dot (QD) with wheat germ agglutinin (WGA) acting as fluorescent and topographic probes prior to cell surface staining is developed for fluorescence microscopy and atomic force microscopy (AFM). This strategy provided at least two advantages: (a) an amplified fluorescence of WGA-QD aggregates, strongly resistant to photobleaching, ensures repeated/real-time observations of the probe-labeled cells by fluorescence microscopy; (b) the enlarged size of WGA-QD probe makes it possible for labeled WGA to be distinguished from other membrane proteins by AFM. Here, the random distribution of WGA-binding sites on non-crosslinked cells and the uneven or polarized reorganization due to WGA-induced crosslinking on cell surfaces were studied using AFM-detectable WGA-QD probe. Moreover, we developed a method to rapidly detect the WGA-induced rigidity alternation of the whole cells, which is efficient and has the potentiality of being developed to a useful tool in clinical diagnosis.
- OSTI ID:
- 21255859
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 379, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2008.12.058; PII: S0006-291X(08)02457-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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