Comparative hepatic cytochrome P450 activities and contaminant concentrations in caged carp and juvenile ducks

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:211952

O`Keefe, P; Gierthy, J; Connor, S; Bush, B; Hong, C S; Wood, L; Clayton, W; Storm, R ^[1]

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1. State Univ. of New York, Albany, NY (United States)

Juvenile carp (Cyprinius carpio) weighing approx. 60 g were placed in cages located on the surface of sediments near an aluminum plant and an automobile parts plant in the Massena area of the St. Lawrence River. Fish were removed at weekly intervals over a 35 day exposure period and composited samples of liver tissue, cranial lipid, and fillet tissue were prepared for analysis of polynuclear aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDDs/PCDFs). Liver tissue was also stored at {minus}80 C for determination of microsomal Cytochrome P450 activity using the aryl hydrocarbon hydroxylase (AHH) assay. A control exposure was carried out upstream at an uncontaminated site. Juvenile pre-flight ducks (mallards, gadwalls, wood ducks and common mergansers) were collected in the contaminated areas on the St. Lawrence and on the Hudson River two to three months after hatching. Control pre-flight mallards, wood ducks and common mergansers were collected from remote lakes in the Addirondack State Park. Samples of subcutaneous fat and liver tissue were removed for analysis as described above for the carp. There was a three fold increase in AHH activity in the carp liver tissue at the end of the 35 day exposure period and there was a similar increase it activity for the mallards, common mergansers and wood ducks compared to controls. For each species the enzyme activity increases will be compared to the contaminant concentrations.

• OSTI ID: 211952
• Report Number(s): CONF-9511137--; ISBN 1-880611-03-1
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BENZOFURANS
BIOLOGICAL ACCUMULATION
COMPARATIVE EVALUATIONS
DIOXIN
ECOLOGICAL CONCENTRATION
ENZYME ACTIVITY
FISHES
HYDROLASES
INDUSTRIAL WASTES
POLYCHLORINATED BIPHENYLS
POLYCYCLIC AROMATIC HYDROCARBONS
SENSITIVITY
ST LAWRENCE RIVER
TISSUE DISTRIBUTION
WATER POLLUTION