Acetylation of the human T-cell leukemia virus type 1 Tax oncoprotein by p300 promotes activation of the NF-{kappa}B pathway

Journal Article · Mon Mar 30 04:00:00 EDT 2009 · Virology

DOI:https://doi.org/10.1016/j.virol.2008.12.043· OSTI ID:21182815

Lodewick, Julie; Lamsoul, Isabelle; Polania, Angela; Lebrun, Sylvie ^[1]; Burny, Arsene ^[2]; Ratner, Lee ^[3]; Bex, Francoise ^[1]

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1. Institute for Microbiological Research J-M Wiame and Laboratory of Microbiology, Universite Libre de Bruxelles, 1, Avenue Emile Gryson, B-1070 Brussels (Belgium)
2. Faculte des Sciences Agronomiques de Gembloux, Gembloux (Belgium)
3. Division of Molecular Oncology, Washington University School of Medicine, St Louis (United States)

The oncogenic potential of the HTLV-1 Tax protein involves activation of the NF-{kappa}B pathway, which depends on Tax phosphorylation, ubiquitination and sumoylation. We demonstrate that the nuclei of Tax-expressing cells, including HTLV-1 transformed T-lymphocytes, contain a pool of Tax molecules acetylated on lysine residue at amino acid position 346 by the transcriptional coactivator p300. Phosphorylation of Tax on serine residues 300/301 was a prerequisite for Tax localization in the nucleus and correlated with its subsequent acetylation by p300, whereas sumoylation, resulting in the formation of Tax nuclear bodies in which p300 was recruited, favored Tax acetylation. Overexpression of p300 markedly increased Tax acetylation and the ability of a wild type HTLV-1 provirus, -but not of a mutant provirus carrying an acetylation deficient Tax gene-, to activate gene expression from an integrated NF-{kappa}B-controlled promoter. Thus, Tax acetylation favors NF-{kappa}B activation and might play an important role in HTLV-1-induced cell transformation.

• OSTI ID: 21182815
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 386; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACETYLATION
CELL TRANSFORMATIONS
GENES
LEUKEMIA VIRUSES
LYMPHOCYTES
LYSINE
MOLECULES
MUTANTS
PHOSPHORYLATION
PROTEINS
SERINE