p21{sup WAF1} modulates NF-{kappa}B signaling and induces anti-apoptotic protein Bcl-2 in Tax-expressing rat fibroblast
- Laboratory of Human Tumor Viruses, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507 (Japan)
Of the cell cycle-associated genes regulated by human T-cell leukemia virus type-1 (HTLV-1) Tax, cyclin-dependent kinase (CDK) inhibitor p21{sup WAF1} is upregulated in HTLV-1-infected cells. Previously, we reported that p21{sup WAF1} stimulated Tax-dependent NF-{kappa}B activation which influences a variety of cellular processes, including proliferation, differentiation, and apoptosis. In HTLV-1-infected cells, Tax is primarily involved in the constitutive activation of NF-{kappa}B signaling. Here, we demonstrate that p21{sup WAF1} affects Tax-dependent NF-{kappa}B signaling by inducing p100/52, an NF-{kappa}B-related protein. W4, a Tax-transformed rat fibroblast cell line, exhibits the constitutive activation of NF-{kappa}B signaling, potentially mediated by overexpression of RelB. Ectopic expression of p21{sup WAF1} in W4 cells, which lack endogenous expression due to methylation of the p21{sup WAF1} promoter, induces the expression of p100/52. Bcl-2 expression was also upregulated by ectopic p21{sup WAF1} in this cell line, suggesting that p21{sup WAF1} plays an important role in the regulation of apoptosis by modulating NF-{kappa}B signaling in Tax-expressing rat fibroblasts. We also address the expression of NF-{kappa}B-related proteins in HTLV-1-infected cells.
- OSTI ID:
- 20637200
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 1 Vol. 332; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
p52-independent nuclear translocation of RelB promotes LPS-induced attachment
Proteasome inhibitors induce apoptosis and reduce viral replication in primary effusion lymphoma cells
Activation of the PI3K-Akt pathway by human T cell leukemia virus type 1 (HTLV-1) oncoprotein Tax increases Bcl3 expression, which is associated with enhanced growth of HTLV-1-infected T cells
Journal Article
·
Thu Dec 31 23:00:00 EST 2009
· Biochemical and Biophysical Research Communications
·
OSTI ID:22199963
Proteasome inhibitors induce apoptosis and reduce viral replication in primary effusion lymphoma cells
Journal Article
·
Thu Dec 01 23:00:00 EST 2011
· Biochemical and Biophysical Research Communications
·
OSTI ID:22207580
Activation of the PI3K-Akt pathway by human T cell leukemia virus type 1 (HTLV-1) oncoprotein Tax increases Bcl3 expression, which is associated with enhanced growth of HTLV-1-infected T cells
Journal Article
·
Sun Aug 01 00:00:00 EDT 2010
· Virology
·
OSTI ID:21460262