Activation of the PI3K-Akt pathway by human T cell leukemia virus type 1 (HTLV-1) oncoprotein Tax increases Bcl3 expression, which is associated with enhanced growth of HTLV-1-infected T cells

Journal Article · Sun Aug 01 04:00:00 EDT 2010 · Virology

DOI:https://doi.org/10.1016/j.virol.2010.04.018· OSTI ID:21460262

Saito, Kousuke ^[1]; Saito, Mineki ^[1]; Taniura, Naoko; Okuwa, Takako; Ohara, Yoshiro ^[1]

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1. Department of Microbiology, Kanazawa Medical University School of Medicine, 1-1 Daigaku, Uchinada-machi, Ishikawa 920-0293 (Japan)

Bcl3 is a member of the I{kappa}B family that regulates genes involved in cell proliferation and apoptosis. Recent reports indicated that Bcl3 is overexpressed in HTLV-1-infected T cells via Tax-mediated transactivation, and acts as a negative regulator of viral transcription. However, the role of Bcl3 in cellular signal transduction and the growth of HTLV-1-infected T cells have not been reported. In this study, we showed that the knockdown of Bcl3 by short hairpin RNA inhibited the growth of HTLV-1-infected T cells. Although phosphatidylinositol-3 kinase (PI3K) inhibitor reduced Bcl3 expression, inactivation of glycogen synthase kinase 3 (GSK3), an effector kinase of the PI3K/Akt signaling pathway, restored Bcl3 expression in Tax-negative but not in Tax-positive T cells. Our results indicate that the overexpression of Bcl3 in HTLV-1-infected T cells is regulated not only by transcriptional but also by post-transcriptional mechanisms, and is involved in overgrowth of HTLV-1-infected T cells.

• OSTI ID: 21460262
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 403; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CELL PROLIFERATION
GENES
GROWTH
LEUKEMIA VIRUSES
MICROORGANISMS
ONCOGENIC VIRUSES
PARASITES
VIRUSES