Chemical compositions responsible for inflammation and tissue damage in the mouse lung by coarse and fine particulate samples from contrasting air pollution in Europe

Journal Article · Tue Jul 01 04:00:00 EDT 2008 · Inhalation Toxicology

DOI:https://doi.org/10.1080/08958370802147282· OSTI ID:21149547

Happo, M S; Hirvonen, M R; Halinen, A I; Jalava, P I; Pennanen, A S; Sillanpaa, M; Hillamo, R; Salonen, R O ^[1]

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1. National Public Health Institute, Kuopio (Finland). Dept. of Environmental Health

Inflammation is regarded as an important mechanism in mortality and morbidity associated with exposures of cardiorespiratory patients to urban air particulate matter. We investigated the association of the chemical composition and sources of urban air fine (PM2.5-0.2) and coarse (PM10-2.5) particulate samples with the inflammatory activity in the mouse lung. The particulate samples were collected during selected seasons in six European cities using a high-volume cascade impactor. Healthy C57BL/6J mice were intratracheally instilled with a single dose (10 mg/kg) of the particulate samples. At 4, 12, and 24 h after the exposure, the lungs were lavaged and the bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation and tissue damage: cell number, total protein, and cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and KC). Dicarboxylic acids and transition metals, especially Ni and V, in PM2.5-0.2 correlated positively and some secondary inorganic ions (NO{sub 3}{sup -}, NH{sub 4}{sup +}) negatively with the inflammatory activity. Total organic matter and SO{sub 4}{sup 2-} had no consistent correlations. In addition, the soil-derived constituents (Ca{sup 2+}, Al, Fe, Si) showed positive correlations with the PM2.5-0.2-induced inflammatory activity, but their role in PM10 (2.5) remained obscure, possibly due to largely undefined biogenic material. Markers of poor biomass and coal combustion, i.e., monosaccharide anhydrides and As, were associated with elevated PAH contents in PM2.5 (0.2) and a consistent immunosuppressive effect. Overall, our results support epidemiological findings that the local sources of incomplete combustion and resuspended road dust are important in urban air particulate pollution-related health effects.

• OSTI ID: 21149547
• Journal Information: Inhalation Toxicology, Journal Name: Inhalation Toxicology Journal Issue: 14 Vol. 20; ISSN INHTE5; ISSN 0895-8378
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
AIR POLLUTION
BIOMASS
CHEMICAL COMPOSITION
COAL
COMBUSTION
DUSTS
HEALTH HAZARDS
INFLAMMATION
INHALATION
LUNGS
MICE
PARTICLE SIZE
PARTICULATES
POLYCYCLIC AROMATIC HYDROCARBONS
ROADS
SOILS
URBAN AREAS
VEHICLES