Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5

Journal Article · Sat Nov 10 04:00:00 EST 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2007.06.017· OSTI ID:21077989

Tran, Kim C ^[1]; He, Biao ^[2]

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1. Department of Biochemistry and Molecular Biology, Pennsylvania State University, 406 South Frear, University Park, PA 16802 (United States)
2. Veterinary and Biomedical Sciences, Center for Molecular Immunology and Infectious Diseases, Pennsylvania State University, University Park, PA 16802 (United States)

Paramyxoviruses have been shown to produce proteins that inhibit interferon production and signaling. For human respiratory syncytial virus (RSV), the nonstructural NS1 and NS2 proteins have been shown to have interferon antagonist activity through an unknown mechanism. To understand further the functions of NS1 and NS2, we generated recombinant RSV in which both NS1 and NS2 were replaced by the PIV5 V protein, which has well-characterized IFN antagonist activities ({delta}NS1/2-V). Expression of V was able to partially inhibit IFN responses in {delta}NS1/2-V-infected cells. In addition, the replication kinetics of {delta}NS1/2-V were intermediate between {delta}NS1/2 and wild-type (rA2) in A549 cells. However, expression of V did not affect the ability of {delta}NS1/2-V to activate IRF3 nuclear translocation and IFN{beta} transcription. These data indicate that V was able to replace some of the IFN inhibitory functions of the RSV NS1 and NS2 proteins, but also that NS1 and NS2 have functions in viral replication beyond IFN antagonism.

• OSTI ID: 21077989
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 368; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
INTERFERON
KINETICS
TRANSCRIPTION
TRANSLOCATION
VIRUSES