hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export
The NS1 protein of influenza viruses is a major virulence factor and exerts its function through interacting with viral/cellular RNAs and proteins. In this study, we identified heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as an interacting partner of NS1 proteins by a proteomic method. Knockdown of hnRNP A2/B1 by small interfering RNA (siRNA) resulted in higher levels of NS vRNA, NS1 mRNA, and NS1 protein in the virus-infected cells. In addition, we demonstrated that hnRNP A2/B1 proteins are associated with NS1 and NS2 mRNAs and that knockdown of hnRNP A2/B1 promotes transport of NS1 mRNA from the nucleus to the cytoplasm in the infected cells. Lastly, we showed that knockdown of hnRNP A2/B1 leads to enhanced virus replication. Our results suggest that hnRNP A2/B1 plays an inhibitory role in the replication of influenza A virus in host cells potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nucleocytoplasmic translocation. - Highlights: • Cellular protein hnRNP A2/B1 interacts with influenza viral protein NS1. • hnRNP A2/B1 suppresses the levels of NS1 protein, vRNA and mRNA in infected cells. • hnRNP A2/B1 protein is associated with NS1 and NS2 mRNAs. • hnRNP A2/B1 inhibits the nuclear export of NS1 mRNAs. • hnRNP A2/B1 inhibits influenza virus replication.
- OSTI ID:
- 22435007
- Journal Information:
- Virology, Journal Name: Virology Vol. 449; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
Direct interaction of cellular hnRNP-F and NS1 of influenza A virus accelerates viral replication by modulation of viral transcriptional activity and host gene expression
Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein
NS1-binding protein abrogates the elevation of cell viability by the influenza A virus NS1 protein in association with CRKL
Journal Article
·
Thu Feb 04 23:00:00 EST 2010
· Virology
·
OSTI ID:21357589
Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein
Journal Article
·
Fri Jul 18 00:00:00 EDT 2008
· Biochemical and Biophysical Research Communications
·
OSTI ID:21143767
NS1-binding protein abrogates the elevation of cell viability by the influenza A virus NS1 protein in association with CRKL
Journal Article
·
Thu Nov 28 23:00:00 EST 2013
· Biochemical and Biophysical Research Communications
·
OSTI ID:22242216