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Title: Radiation Interaction with Therapeutic Drugs and Cell Membranes

Abstract

This transient permeabilized state of the cell membrane, named the 'cell electroporation' (CE) can be used to increase cells uptake of drugs that do not readily pass cell membrane, thus enabling their cytotoxicity. The anticancer drugs, such as bleomycin (BL) and cisplatin, are the most candidates for the combined use with ionizing and non-ionizing radiation fields. The methods and installations for the cell electroporation by electron beam (EB) and microwave (MW) irradiation are presented. The viability tests of the human leukocytes under EB and MW exposure with/without the BL in the cell cultures are discussed.

Authors:
; ; ; ; ;  [1]; ; ;  [2];  [3];  [4]
  1. Accelerators Laboratory, National Institute for Lasers, Plasma and Radiation Physics, 409, Atomistilor St., 077125 Magurele (Romania)
  2. Carol Davila University of Medicine and Pharmacy, 8, Eroii Sanitari St., 050474 Bucharest (Romania)
  3. Oncology Institute Prof. A. Trestioreanu, 252, Fundeni St., 022328 Bucharest (Romania)
  4. Military Clinical Hospital, 88, Mircea Vulcanescu St., 010825 Bucharest (Romania)
Publication Date:
OSTI Identifier:
21057291
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 899; Journal Issue: 1; Conference: 6. international conference of the Balkan Physical Union, Istanbul (Turkey), 22-26 Aug 2006; Other Information: DOI: 10.1063/1.2733557; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLEOMYCIN; CELL CULTURES; CELL MEMBRANES; ELECTRON BEAMS; HUMAN POPULATIONS; IONIZING RADIATIONS; IRRADIATION; LEUKOCYTES; MICROWAVE RADIATION; NEOPLASMS; TRANSIENTS; UPTAKE

Citation Formats

Martin, Diana I., Manaila, Elena N., Matei, Constantin I., Iacob, Nicusor I., Ighigeanu, Daniel I., Craciun, Gabriela D., Moisescu, Mihaela I., Savopol, Tudor D., Kovacs, Eugenia A., Cinca, Sabin A., and Margaritescu, Irina D.. Radiation Interaction with Therapeutic Drugs and Cell Membranes. United States: N. p., 2007. Web. doi:10.1063/1.2733557.
Martin, Diana I., Manaila, Elena N., Matei, Constantin I., Iacob, Nicusor I., Ighigeanu, Daniel I., Craciun, Gabriela D., Moisescu, Mihaela I., Savopol, Tudor D., Kovacs, Eugenia A., Cinca, Sabin A., & Margaritescu, Irina D.. Radiation Interaction with Therapeutic Drugs and Cell Membranes. United States. doi:10.1063/1.2733557.
Martin, Diana I., Manaila, Elena N., Matei, Constantin I., Iacob, Nicusor I., Ighigeanu, Daniel I., Craciun, Gabriela D., Moisescu, Mihaela I., Savopol, Tudor D., Kovacs, Eugenia A., Cinca, Sabin A., and Margaritescu, Irina D.. Mon . "Radiation Interaction with Therapeutic Drugs and Cell Membranes". United States. doi:10.1063/1.2733557.
@article{osti_21057291,
title = {Radiation Interaction with Therapeutic Drugs and Cell Membranes},
author = {Martin, Diana I. and Manaila, Elena N. and Matei, Constantin I. and Iacob, Nicusor I. and Ighigeanu, Daniel I. and Craciun, Gabriela D. and Moisescu, Mihaela I. and Savopol, Tudor D. and Kovacs, Eugenia A. and Cinca, Sabin A. and Margaritescu, Irina D.},
abstractNote = {This transient permeabilized state of the cell membrane, named the 'cell electroporation' (CE) can be used to increase cells uptake of drugs that do not readily pass cell membrane, thus enabling their cytotoxicity. The anticancer drugs, such as bleomycin (BL) and cisplatin, are the most candidates for the combined use with ionizing and non-ionizing radiation fields. The methods and installations for the cell electroporation by electron beam (EB) and microwave (MW) irradiation are presented. The viability tests of the human leukocytes under EB and MW exposure with/without the BL in the cell cultures are discussed.},
doi = {10.1063/1.2733557},
journal = {AIP Conference Proceedings},
number = 1,
volume = 899,
place = {United States},
year = {Mon Apr 23 00:00:00 EDT 2007},
month = {Mon Apr 23 00:00:00 EDT 2007}
}
  • This paper presents a summary of the morphological categorization of cell death, results of two in vivo studies on the cell death induced by mild hyperthermia in rat small intestine and mouse mastocytoma, and a comparison of the cell death induced by hyperthermia, radiation and cytotoxic drugs. Two distinct forms of cell death, apoptosis and necrosis, can be recognized on morphologic grounds. Apoptosis appears to be a process of active cellular self-destruction to which a biologically meaningful role can usually be attributed, whereas necrosis is a passive degenerative phenomenon that results from irreversible cellular injury. Light and transmission electron microscopicmore » studies showed that lower body hyperthermia (43 degrees C for 30 min) induced only apoptosis of intestinal epithelial cells, and of lymphocytes, plasma cells, and eosinophils. In the mastocytoma, hyperthermia (43 degrees C for 15 min) produced widespread tumor necrosis and also enhanced apoptosis of tumor cells. Ionizing radiation and cytotoxic drugs are also known to induce apoptosis in a variety of tissues. It is attractive to speculate that DNA damage by each agent is the common event which triggers the same process of active cellular self-destruction that characteristically effects selective cell deletion in normal tissue homeostasis.« less
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  • Drug-resistant mammalian tumor cell lines have been derived by either fractionated x-irradiation treatment or exposure to vincristine or etoposide (VP-16-213) in vitro. Analyses of the patterns of responses expressed by these differently derived, resistant cell lines have shown variations in responses to a range of antitumor drugs depending upon the agent used to induce resistance. However, all treated cell lines express resistance to vincristine and, with one exception, to VP-16-213. Preliminary evidence has indicated that resistance to vincristine in drug-treated cells, but not x-irradiation-treated cells, is associated with impaired vincristine uptake; resistance to VP-16-213 in both differently derived, resistant sublinesmore » is associated with a reduction of VP-16-213-induced DNA single-strand breakage; and collateral sensitivity to cisplatin in x-irradiation-treated cells is associated with enhanced drug-induced DNA cross-linking. These data indicate that patterns of responses to antitumor drugs and the mechanisms associated with these altered responses differ depending upon the agent used to induce resistance.« less