Anaerobic transformations of carbon tetrachloride: Combined bacterial and abiotic processes

Conference · Sun Dec 31 04:00:00 UTC 1995

OSTI ID:210541

Kim, S; Picardal, F W; Radue, A ^[1]

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1. Indiana Univ., Bloomington, IN (United States); and others

Shewanella putrefaciens 200, is able to dechlorinate carbon tetrachloride (CT) under anaerobic conditions and served as a model bacterium for examining the role of inorganic and organic soil/sediment components on rates of CT transformation. Pseudo-first-order rate constants for CT degradation increased 10-fold in the presence of 150 mM Fe(III)-oxide and were linearly proportional to Fe(Ill)-oxide concentrations. The Fe(III)-oxide did not transform CT in the absence of S. putrefaciens. CT dechlorination in microbial batch reactors was also increased by the presence of soil containing a high concentration of natural organic matter. The same soil catalyzed CT dechlorination in the absence of S. putrefaciens when dithiothreitol was added as a reductant. This work suggests that the relative contribution of (i) direct microbial transformations and (ii) microbially mediated abiotic transformations by inorganic or organic sediment catalysts will vary with sediment composition. Increased biotreatment rates may be possible by utilization of sediment amendments that increase abiotic transformation rates.

• OSTI ID: 210541
• Report Number(s): CONF-9509139--
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
ANAEROBIC DIGESTION
BIOCHEMICAL REACTION KINETICS
CARBON TETRACHLORIDE
DECONTAMINATION
SOILS