Transformation of tetrachloromethane by Shewanella putrefaciens MR-1

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:474263

Petrovskis, E A; Adriaens, P ^[1]; Vogel, T M ^[2]; Saffarini, D A; Nealson, K H ^[3]

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1. Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering
2. Rhone-Poulenc Industrialisation, Decines (France)
3. Univ. of Wisconsin, Milwaukee, WI (United States). Center for Great Lakes Studies

The dissimilatory metal-reducing facultative anaerobe Shewanella putrefaciens MR-1 has been shown to transform chlorinated methanes by biotic and abiotic processes under Fe(III)-respiring conditions. MR-1 produces magnetite and other Fe(II)-containing compounds that dehalogenate tetrachloromethane (CT) and trichloromethane (CF) abiotically. The biological CT transformation activity has been localized utilizing metabolic inhibitors and respiratory mutants. Cytochrome and iron-sulfur complex inhibitors did not block CT transformation. Terminal reductase mutants showed wild-type CT transformation activity. However, menaquinone-deficient mutants lost 90% of the CT transformation activity. This represents the first direct evidence of respiratory chain involvement in reductive dehalogenation.

• OSTI ID: 474263
• Report Number(s): CONF-950483--; ISBN 1-57477-005-5
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
CHLORINATED ALIPHATIC HYDROCARBONS
ENZYME ACTIVITY
IN-SITU PROCESSING
METABOLISM
REMEDIAL ACTION