Molecular cloning and developmental expression of plakophilin 2 in zebrafish

Journal Article · Fri Feb 29 04:00:00 EST 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.12.106· OSTI ID:21043637

Moriarty, Miriam A; Martin, Eva D ^[1]; Byrnes, Lucy ^[2]; Grealy, Maura ^[1]

---

1. Department of Pharmacology and Therapeutics and National Centre for Biomedical Engineering Science, National University of Ireland, University Road, Galway (Ireland)
2. Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland, Galway (Ireland)

Armadillo proteins are involved in providing strength and support to cells and tissues, nuclear transport, and transcriptional activation. In this report, we describe the identification and characterisation of the cDNA of the desmosomal armadillo protein plakophilin 2 in zebrafish. The 2448 bp coding sequence encodes a predicted 815 amino acid protein, with nine armadillo repeats characteristic of the p120-catenin subfamily. It shares conserved N-glycosylation, myristoylation, and glycogen synthase kinase 3, casein kinase 2, and protein kinase C phosphorylation sites with mammalian armadillo proteins including plakoglobin and {beta}-catenin. Semi-quantitative reverse transcription polymerase chain reaction and whole mount in situ hybridisation show that it is expressed both maternally and zygotically. It is ubiquitously expressed during blastula stages but becomes restricted to epidermal and cardiac tissue during gastrulation. These results provide evidence that zebrafish plakophilin 2 is developmentally regulated with potential roles in cell adhesion, signalling, and cardiac and skin development.

• OSTI ID: 21043637
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 367; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AMINO ACIDS
CASEIN
CLONING
GLYCOGEN
PHOSPHORYLATION
POLYMERASE CHAIN REACTION
SKIN
TRANSCRIPTION