The RSV F and G glycoproteins interact to form a complex on the surface of infected cells
- School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore)
- Detection and Diagnostics Laboratory, Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, 13-00, Singapore 117510 (Singapore)
In this study, the interaction between the respiratory syncytial virus (RSV) fusion (F) protein, attachment (G) protein, and small hydrophobic (SH) proteins was examined. Immunoprecipitation analysis suggested that the F and G proteins exist as a protein complex on the surface of RSV-infected cells, and this conclusion was supported by ultracentrifugation analysis that demonstrated co-migration of surface-expressed F and G proteins. Although our analysis provided evidence for an interaction between the G and SH proteins, no evidence was obtained for a single protein complex involving all three of the virus proteins. These data suggest the existence of multiple virus glycoprotein complexes within the RSV envelope. Although the stimulus that drives RSV-mediated membrane fusion is unknown, the association between the G and F proteins suggest an indirect role for the G protein in this process.
- OSTI ID:
- 21043609
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 366, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.11.042; PII: S0006-291X(07)02456-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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