Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies

Jones, Harrison G.; Ritschel, Tina; Pascual, Gabriel; Brakenhoff, Just P. J.; Keogh, Elissa; Furmanova-Hollenstein, Polina; Lanckacker, Ellen; Wadia, Jehangir S.; Gilman, Morgan S. A.; Williamson, R. Anthony; Roymans, Dirk; van ‘t Wout, Angélique B.; Langedijk, Johannes P.; McLellan, Jason S.; Pierson, Ted C.

doi:10.1371/journal.ppat.1006935

Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies

Journal Article · Mon Mar 05 23:00:00 EST 2018 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1006935· OSTI ID:1484790

Jones, Harrison G. ^[1]; ^[2]; ^[3]; Brakenhoff, Just P. J. ^[2]; ^[3]; Furmanova-Hollenstein, Polina ^[2]; ^[4]; Wadia, Jehangir S. ^[5]; Gilman, Morgan S. A. ^[1]; ^[6]; Roymans, Dirk ^[4]; ^[7]; ^[2]; ^[1]; Pierson, Ted C. ^[8]

Univ. of Texas, Austin, TX (United States); Geisel School of Medicine at Dartmouth, Hanover, NH (United States)
Janssen Vaccines & Prevention, Leiden (The Netherlands)
Janssen Pharmaceutical Companies of Johnson and Johnson, San Diego, CA (United States)
Janssen Pharmaceutica NV, Beerse (Belgium)
Janssen R&D US, San Diego, CA (United States)
Janssen Pharmaceutical Companies of Johnson and Johnson, London (United Kingdom)
Janssen Vaccines & Prevention B.V., Leiden (The Netherlands)
National Inst. of Health (NIH), Bethesda, MD (United States)

Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants and the elderly, and yet there remains no effective treatment or vaccine. The surface of the virion is decorated with the fusion glycoprotein (RSV F) and the attachment glycoprotein (RSV G), which binds to CX3CR1 on human airway epithelial cells to mediate viral attachment and subsequent infection. RSV G is a major target of the humoral immune response, and antibodies that target the central conserved region of G have been shown to neutralize both subtypes of RSV and to protect against severe RSV disease in animal models. However, the molecular underpinnings for antibody recognition of this region have remained unknown. Therefore, we isolated two human antibodies directed against the central conserved region of RSV G and demonstrated that they neutralize RSV infection of human bronchial epithelial cell cultures in the absence of complement. Moreover, the antibodies protected cotton rats from severe RSV disease. Both antibodies bound with high affinity to a secreted form of RSV G as well as to a peptide corresponding to the unglycosylated central conserved region. High-resolution crystal structures of each antibody in complex with the G peptide revealed two distinct conformational epitopes that require proper folding of the cystine noose located in the C-terminal part of the central conserved region. Comparison of these structures with the structure of fractalkine (CX3CL1) alone or in complex with a viral homolog of CX3CR1 (US28) suggests that RSV G would bind to CX3CR1 in a mode that is distinct from that of fractalkine. Collectively, these results build on recent studies demonstrating the importance of RSV G in antibody-mediated protection from severe RSV disease, and the structural information presented here should guide the development of new vaccines and antibody-based therapies for RSV.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Scientific User Facilities Division; NIH, Office of Research Infrastructure Programs

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1484790

Journal Information:: PLoS Pathogens, Journal Name: PLoS Pathogens Journal Issue: 3 Vol. 14; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (84)

Risk of Primary Infection and Reinfection With Respiratory Syncytial Virus Glezen, W. Paul Archives of Pediatrics & Adolescent Medicine, Vol. 140, Issue 6 https://doi.org/10.1001/archpedi.1986.02140200053026	journal	June 1986
Comparison of affinity chromatography and adsorption to vaccinia virus recombinant infected cells for depletion of antibodies directed against respiratory syncytial virus glycoproteins present in a human immunoglobulin preparation: Comparison of Alternate Methods for Adsorption of Anti-RSV Neutralizing Antibodies Sastre, Patricia; Melero, José A.; García-Barreno, Blanca Journal of Medical Virology, Vol. 76, Issue 2 https://doi.org/10.1002/jmv.20349	journal	April 2005
Host Cell Effect upon Glycosylation and Antigenicity of Human Respiratory Syncytial Virus G Glycoprotein GarcÍA-Beato, Regina; MartÍNez, Isidoro; FrancÍ, Clara Virology, Vol. 221, Issue 2 https://doi.org/10.1006/viro.1996.0379	journal	July 1996
Iduronic Acid-Containing Glycosaminoglycans on Target Cells Are Required for Efficient Respiratory Syncytial Virus Infection Hallak, Louay K.; Collins, Peter L.; Knudson, Warren Virology, Vol. 271, Issue 2 https://doi.org/10.1006/viro.2000.0293	journal	June 2000
Contribution of the Respiratory Syncytial Virus G Glycoprotein and Its Secreted and Membrane-Bound Forms to Virus Replication in Vitro and in Vivo Teng, Michael N.; Whitehead, Stephen S.; Collins, Peter L. Virology, Vol. 289, Issue 2 https://doi.org/10.1006/viro.2001.1138	journal	October 2001
The fusion and hemagglutinin-neuraminidase glycoproteins of human parainfluenza virus 3 are both required for fusion Ebata, Sharon N.; Côté, Marie-José; Yong Kang, C. Virology, Vol. 183, Issue 1 https://doi.org/10.1016/0042-6822(91)90162-5	journal	July 1991
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Structural, antigenic and immunogenic features of respiratory syncytial virus glycoproteins relevant for vaccine development Melero, José A.; Mas, Vicente; McLellan, Jason S. Vaccine, Vol. 35, Issue 3 https://doi.org/10.1016/j.vaccine.2016.09.045	journal	January 2017
Identification and Molecular Characterization of Fractalkine Receptor CX3CR1, which Mediates Both Leukocyte Migration and Adhesion Imai, Toshio; Hieshima, Kunio; Haskell, Christopher Cell, Vol. 91, Issue 4 https://doi.org/10.1016/s0092-8674(00)80438-9	journal	November 1997
Solution Structure of the Immunodominant Region of Protein G of Bovine Respiratory Syncytial Virus ^† ^, ^‡ Doreleijers, Jurgen F.; Langedijk, Johannes P. M.; Hård, Karl Biochemistry, Vol. 35, Issue 47 https://doi.org/10.1021/bi9621627	journal	January 1996
CX3C chemokine mimicry by respiratory syncytial virus G glycoprotein Tripp, Ralph A.; Jones, Les P.; Haynes, Lia M. Nature Immunology, Vol. 2, Issue 8 https://doi.org/10.1038/90675	journal	August 2001
Therapeutic efficacy of a respiratory syncytial virus fusion inhibitor Roymans, Dirk; Alnajjar, Sarhad S.; Battles, Michael B. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00170-x	journal	August 2017
The Burden of Respiratory Syncytial Virus Infection in Young Children Hall, Caroline Breese; Weinberg, Geoffrey A.; Iwane, Marika K. New England Journal of Medicine, Vol. 360, Issue 6 https://doi.org/10.1056/nejmoa0804877	journal	February 2009
In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo Villenave, R.; Thavagnanam, S.; Sarlang, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 13 https://doi.org/10.1073/pnas.1110203109	journal	March 2012
The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Johnson, P. R.; Spriggs, M. K.; Olmsted, R. A. Proceedings of the National Academy of Sciences, Vol. 84, Issue 16 https://doi.org/10.1073/pnas.84.16.5625	journal	August 1987
Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant Karron, R. A.; Buonagurio, D. A.; Georgiu, A. F. Proceedings of the National Academy of Sciences, Vol. 94, Issue 25 https://doi.org/10.1073/pnas.94.25.13961	journal	December 1997
The Crystal Structure of the Chemokine Domain of Fractalkine Shows a Novel Quaternary Arrangement Hoover, David M.; Mizoue, Laura S.; Handel, Tracy M. Journal of Biological Chemistry, Vol. 275, Issue 30 https://doi.org/10.1074/jbc.m002584200	journal	April 2000
Therapeutic Monoclonal Antibody Treatment Targeting Respiratory Syncytial Virus (RSV) G Protein Mediates Viral Clearance and Reduces the Pathogenesis of RSV Infection in BALB/c Mice Haynes, Lia M.; Caidi, Hayat; Radu, Gertrud U. The Journal of Infectious Diseases, Vol. 200, Issue 3 https://doi.org/10.1086/600108	journal	August 2009
Antibodies to the Central Conserved Region of Respiratory Syncytial Virus (RSV) G Protein Block RSV G Protein CX3C-CX3CR1 Binding and Cross-Neutralize RSV A and B Strains Choi, Youngjoo; Mason, Caleb S.; Jones, Les P. Viral Immunology https://doi.org/10.1089/vim.2011.0094	journal	May 2012
Respiratory syncytial virus envelope glycoprotein (G) has a novel structure Satake, Masanobu; Coligan, John E.; Elango, Narayanasamy Nucleic Acids Research, Vol. 13, Issue 21 https://doi.org/10.1093/nar/13.21.7795	journal	January 1985
Appearance of a Soluble Form of the G Protein of Respiratory Syncytial Virus in Fluids of Infected Cells Hendricks, D. A.; Baradaran, K.; McIntosh, K. Journal of General Virology, Vol. 68, Issue 6 https://doi.org/10.1099/0022-1317-68-6-1705	journal	June 1987
Demonstration that Glycoprotein G Is the Attachment Protein of Respiratory Syncytial Virus Levine, S.; Klaiber-Franco, R.; Paradiso, P. R. Journal of General Virology, Vol. 68, Issue 9 https://doi.org/10.1099/0022-1317-68-9-2521	journal	September 1987
Comparison of Antigenic Sites of Subtype-specific Respiratory Syncytial Virus Attachment Proteins Walsh, E. E.; Hall, C. B.; Schlesinger, J. J. Journal of General Virology, Vol. 70, Issue 11 https://doi.org/10.1099/0022-1317-70-11-2953	journal	November 1989
Oligomerization and post-translational processing of glycoprotein G of human respiratory syncytial virus: altered O-glycosylation in the presence of brefeldin A Collins, P. L.; Mottet, G. Journal of General Virology, Vol. 73, Issue 4 https://doi.org/10.1099/0022-1317-73-4-849	journal	April 1992
Proposed three-dimensional model for the attachment protein G of respiratory syncytial virus Langedijk, J. P. M.; Schaaper, W. M. M.; Meloen, R. H. Journal of General Virology, Vol. 77, Issue 6 https://doi.org/10.1099/0022-1317-77-6-1249	journal	June 1996
CX3CR1 is an important surface molecule for respiratory syncytial virus infection in human airway epithelial cells Stobart, Christopher C.; Hartert, Tina V.; Lin, Songbai Journal of General Virology, Vol. 96, Issue 9 https://doi.org/10.1099/vir.0.000218	journal	September 2015
Treatment with respiratory syncytial virus G glycoprotein monoclonal antibody or F(ab')2 components mediates reduced pulmonary inflammation in mice Miao, C.; Radu, G. U.; Caidi, H. Journal of General Virology, Vol. 90, Issue 5 https://doi.org/10.1099/vir.0.009308-0	journal	March 2009
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/s0907444904019158	journal	November 2004
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/s0907444910045749	journal	March 2011
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/s0907444910048675	journal	March 2011
Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor Burg, J. S.; Ingram, J. R.; Venkatakrishnan, A. J. Science, Vol. 347, Issue 6226 https://doi.org/10.1126/science.aaa5026	journal	March 2015
Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera Ngwuta, Joan O.; Chen, Man; Modjarrad, Kayvon Science Translational Medicine, Vol. 7, Issue 309 https://doi.org/10.1126/scitranslmed.aac4241	journal	October 2015
Protection against a mixed SHIV challenge by a broadly neutralizing antibody cocktail Julg, Boris; Liu, Po-Ting; Wagh, Kshitij Science Translational Medicine, Vol. 9, Issue 408 https://doi.org/10.1126/scitranslmed.aao4235	journal	September 2017
Identification of epitopes on respiratory syncytial virus proteins by competitive binding immunoassay. Anderson, L. J.; Hierholzer, J. C.; Stone, Y. O. Journal of Clinical Microbiology, Vol. 23, Issue 3 https://doi.org/10.1128/jcm.23.3.475-480.1986	journal	January 1986
Respiratory Syncytial Virus G Protein CX3C Motif Impairs Human Airway Epithelial and Immune Cell Responses Chirkova, T.; Boyoglu-Barnum, S.; Gaston, K. A. Journal of Virology, Vol. 87, Issue 24 https://doi.org/10.1128/jvi.01741-13	journal	October 2013
Broadly Reactive Anti-Respiratory Syncytial Virus G Antibodies from Exposed Individuals Effectively Inhibit Infection of Primary Airway Epithelial Cells Cortjens, B.; Yasuda, E.; Yu, X. Journal of Virology, Vol. 91, Issue 10 https://doi.org/10.1128/jvi.02357-16	journal	March 2017
The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons. Roberts, S. R.; Lichtenstein, D.; Ball, L. A. Journal of Virology, Vol. 68, Issue 7 https://doi.org/10.1128/jvi.68.7.4538-4546.1994	journal	January 1994
Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G Feldman, Steven A.; Hendry, R. Michael; Beeler, Judy A. Journal of Virology, Vol. 73, Issue 8 https://doi.org/10.1128/jvi.73.8.6610-6617.1999	journal	August 1999
Glycosaminoglycan Sulfation Requirements for Respiratory Syncytial Virus Infection Hallak, L. K.; Spillmann, D.; Collins, P. L. Journal of Virology, Vol. 74, Issue 22 https://doi.org/10.1128/jvi.74.22.10508-10513.2000	journal	November 2000
Recombinant Human Metapneumovirus Lacking the Small Hydrophobic SH and/or Attachment G Glycoprotein: Deletion of G Yields a Promising Vaccine Candidate Biacchesi, S.; Skiadopoulos, M. H.; Yang, L. Journal of Virology, Vol. 78, Issue 23 https://doi.org/10.1128/jvi.78.23.12877-12887.2004	journal	November 2004
Effectiveness of Palivizumab in Preventing RSV Hospitalization in High Risk Children: A Real-World Perspective Homaira, Nusrat; Rawlinson, William; Snelling, Thomas L. International Journal of Pediatrics, Vol. 2014 https://doi.org/10.1155/2014/571609	journal	January 2014
Effects of Anti-G and Anti-F Antibodies on Airway Function after Respiratory Syncytial Virus Infection Han, Junyan; Takeda, Katsuyuki; Wang, Meiqin American Journal of Respiratory Cell and Molecular Biology, Vol. 51, Issue 1 https://doi.org/10.1165/rcmb.2013-0360oc	journal	July 2014
The Respiratory Syncytial Virus G Protein Conserved Domain Induces a Persistent and Protective Antibody Response in Rodents Nguyen, Thien N.; Power, Ultan F.; Robert, Alain PLoS ONE, Vol. 7, Issue 3 https://doi.org/10.1371/journal.pone.0034331	journal	March 2012
CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes with Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner Jeong, Kwang-Il; Piepenhagen, Peter A.; Kishko, Michael PLOS ONE, Vol. 10, Issue 6 https://doi.org/10.1371/journal.pone.0130517	journal	June 2015
X-Ray Diffraction data from RSV G peptide bound to Fab CB002.5, source of 6BLI structure Jones, Harrison G. SBGrid Data Bank https://doi.org/10.15785/sbgrid/635	dataset	January 2018
X-Ray Diffraction data from RSV G peptide bound to Fab CB017.5, source of 6BLH structure Jones, Harrison G. SBGrid Data Bank https://doi.org/10.15785/sbgrid/636	dataset	January 2018
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Potent High-Affinity Antibodies for Treatment and Prophylaxis of Respiratory Syncytial Virus Derived from B Cells of Infected Patients Collarini, Ellen J.; Lee, F. Eun-Hyung; Foord, Orit The Journal of Immunology, Vol. 183, Issue 10 https://doi.org/10.4049/jimmunol.0901373	journal	October 2009
Determination of the disulfide bond arrangement of human respiratory syncytial virus attachment (G) protein by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: Disuljides of respiratory syncytial virus G protein Gorman, Jeffrey J.; Ferguson, Bettina L.; Speelman, Dan Protein Science, Vol. 6, Issue 6 https://doi.org/10.1002/pro.5560060619	journal	June 1997
Circulation of human coronaviruses OC43 and 229E in Córdoba, Argentina Ottogalli, María Emilia; Rodríguez, Pamela Elizabeth; Frutos, María Celia Archives of Virology, Vol. 166, Issue 3 https://doi.org/10.1007/s00705-020-04914-x	journal	January 2021
Identification and Molecular Characterization of Fractalkine Receptor CX3CR1, which Mediates Both Leukocyte Migration and Adhesion Imai, Toshio; Hieshima, Kunio; Haskell, Christopher Cell, Vol. 91, Issue 4 https://doi.org/10.1016/S0092-8674(00)80438-9	journal	November 1997
Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study Shi, Ting; McAllister, David A.; O'Brien, Katherine L. The Lancet, Vol. 390, Issue 10098 https://doi.org/10.1016/S0140-6736(17)30938-8	journal	September 2017
Structure-antigenicity relationship studies of the central conserved region of human respiratory syncytial virus protein G Sugawara, M.; Czaplicki, J.; Ferrage, J. Journal of Peptide Research, Vol. 60, Issue 5 https://doi.org/10.1034/j.1399-3011.2002.21027.x	journal	November 2002
Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins Mukherjee, Somnath; Erramilli, Satchal K.; Ammirati, Mark Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15363-0	journal	March 2020
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
Respiratory Syncytial Virus Infection in Elderly and High-Risk Adults Falsey, Ann R.; Hennessey, Patricia A.; Formica, Maria A. New England Journal of Medicine, Vol. 352, Issue 17 https://doi.org/10.1056/NEJMoa043951	journal	April 2005
The Burden of Respiratory Syncytial Virus Infection in Young Children Hall, Caroline Breese; Weinberg, Geoffrey A.; Iwane, Marika K. New England Journal of Medicine, Vol. 360, Issue 6 https://doi.org/10.1056/NEJMoa0804877	journal	February 2009
Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Wertz, G. W.; Collins, P. L.; Huang, Y. Proceedings of the National Academy of Sciences, Vol. 82, Issue 12 https://doi.org/10.1073/pnas.82.12.4075	journal	June 1985
The Crystal Structure of the Chemokine Domain of Fractalkine Shows a Novel Quaternary Arrangement Hoover, David M.; Mizoue, Laura S.; Handel, Tracy M. Journal of Biological Chemistry, Vol. 275, Issue 30 https://doi.org/10.1074/jbc.M002584200	journal	April 2000
Measles virus: both the haemagglutinin and fusion glycoproteins are required for fusion Wild, T. F.; Malvoisin, E.; Buckland, R. Journal of General Virology, Vol. 72, Issue 2 https://doi.org/10.1099/0022-1317-72-2-439	journal	February 1991
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910048675	journal	March 2011
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Identification of epitopes on respiratory syncytial virus proteins by competitive binding immunoassay. Anderson, L. J.; Hierholzer, J. C.; Stone, Y. O. Journal of Clinical Microbiology, Vol. 23, Issue 3 https://doi.org/10.1128/JCM.23.3.475-480.1986	journal	January 1986
Structural Insights into the Human Metapneumovirus Glycoprotein Ectodomain Leyrat, C.; Paesen, G. C.; Charleston, J. Journal of Virology, Vol. 88, Issue 19 https://doi.org/10.1128/JVI.01726-14	journal	July 2014
Respiratory Syncytial Virus G Protein CX3C Motif Impairs Human Airway Epithelial and Immune Cell Responses Chirkova, T.; Boyoglu-Barnum, S.; Gaston, K. A. Journal of Virology, Vol. 87, Issue 24 https://doi.org/10.1128/JVI.01741-13	journal	October 2013
Vaccination To Induce Antibodies Blocking the CX3C-CX3CR1 Interaction of Respiratory Syncytial Virus G Protein Reduces Pulmonary Inflammation and Virus Replication in Mice Zhang, W.; Choi, Y.; Haynes, L. M. Journal of Virology, Vol. 84, Issue 2 https://doi.org/10.1128/JVI.01755-09	journal	October 2009
Broadly Reactive Anti-Respiratory Syncytial Virus G Antibodies from Exposed Individuals Effectively Inhibit Infection of Primary Airway Epithelial Cells Cortjens, B.; Yasuda, E.; Yu, X. Journal of Virology, Vol. 91, Issue 10 https://doi.org/10.1128/JVI.02357-16	journal	March 2017
Fusion properties of cells persistently infected with human parainfluenza virus type 3: participation of hemagglutinin-neuraminidase in membrane fusion. Moscona, A.; Peluso, R. W. Journal of Virology, Vol. 65, Issue 6 https://doi.org/10.1128/JVI.65.6.2773-2777.1991	journal	January 1991
The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons. Roberts, S. R.; Lichtenstein, D.; Ball, L. A. Journal of Virology, Vol. 68, Issue 7 https://doi.org/10.1128/JVI.68.7.4538-4546.1994	journal	January 1994
Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G Feldman, Steven A.; Hendry, R. Michael; Beeler, Judy A. Journal of Virology, Vol. 73, Issue 8 https://doi.org/10.1128/JVI.73.8.6610-6617.1999	journal	August 1999
Glycosaminoglycan Sulfation Requirements for Respiratory Syncytial Virus Infection Hallak, L. K.; Spillmann, D.; Collins, P. L. Journal of Virology, Vol. 74, Issue 22 https://doi.org/10.1128/JVI.74.22.10508-10513.2000	journal	November 2000
Recombinant Human Metapneumovirus Lacking the Small Hydrophobic SH and/or Attachment G Glycoprotein: Deletion of G Yields a Promising Vaccine Candidate Biacchesi, S.; Skiadopoulos, M. H.; Yang, L. Journal of Virology, Vol. 78, Issue 23 https://doi.org/10.1128/JVI.78.23.12877-12887.2004	journal	November 2004
Infection of Nonhuman Primates with Recombinant Human Metapneumovirus Lacking the SH, G, or M2-2 Protein Categorizes Each as a Nonessential Accessory Protein and Identifies Vaccine Candidates Biacchesi, S.; Pham, Q. N.; Skiadopoulos, M. H. Journal of Virology, Vol. 79, Issue 19 https://doi.org/10.1128/JVI.79.19.12608-12613.2005	journal	September 2005
Infection of Ciliated Cells by Human Parainfluenza Virus Type 3 in an In Vitro Model of Human Airway Epithelium Zhang, L.; Bukreyev, A.; Thompson, C. I. Journal of Virology, Vol. 79, Issue 2 https://doi.org/10.1128/JVI.79.2.1113-1124.2005	journal	December 2004
Effects of Anti-G and Anti-F Antibodies on Airway Function after Respiratory Syncytial Virus Infection Han, Junyan; Takeda, Katsuyuki; Wang, Meiqin American Journal of Respiratory Cell and Molecular Biology, Vol. 51, Issue 1 https://doi.org/10.1165/rcmb.2013-0360OC	journal	July 2014
Solution Structure of the Immunodominant Region of Protein G of Bovine Respiratory Syncytial Virus Doreleijers, Jurgen F.; Langedijk, Johannes PM.; Herd, Karl Biological Magnetic Resonance Bank https://doi.org/10.13018/bmr4020	dataset	January 2003
Respiratory Syncytial Virus Uses CX3CR1 as a Receptor on Primary Human Airway Epithelial Cultures Johnson, Sara M.; McNally, Beth A.; Ioannidis, Ioannis PLOS Pathogens, Vol. 11, Issue 12 https://doi.org/10.1371/journal.ppat.1005318	journal	December 2015
Infection of Ciliated Cells by Human Parainfluenza Virus Type 3 in an In Vitro Model of Human Airway Epithelium A., Bukreyev,; J., Pickles, R.; B., Watson, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/bgjp-y127	text	January 2005
Overview of the CCP4 suite and current developments. Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.52322	text	January 2011
Collaboration gets the most out of software Morin, Andrew; Eisenbraun, Ben; Key, Jason eLife, Vol. 2 https://doi.org/10.7554/eLife.01456	journal	September 2013

Cited By (7)

Respiratory syncytial virus entry and how to block it Battles, Michael B.; McLellan, Jason S. Nature Reviews Microbiology, Vol. 17, Issue 4 https://doi.org/10.1038/s41579-019-0149-x	journal	February 2019
Antibody Epitopes of Pneumovirus Fusion Proteins Huang, Jiachen; Diaz, Darren; Mousa, Jarrod J. Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.02778	journal	November 2019
Development of Luciferase Immunoprecipitation Systems (LIPS) Assay to Detect IgG Antibodies against Human Respiratory Syncytial Virus G-Glycoprotein Crim, Roberta; Kumari, Sangeeta; Jayanti, Priyanka Vaccines, Vol. 7, Issue 1 https://doi.org/10.3390/vaccines7010016	journal	February 2019
Respiratory syncytial virus reduces STAT3 phosphorylation in human memory CD8 T cells stimulated with IL-21 Antunes, Krist Helen; Becker, André; Franceschina, Caroline Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-54240-9	journal	November 2019
Effects of Alterations to the CX3C Motif and Secreted Form of Human Respiratory Syncytial Virus (RSV) G Protein on Immune Responses to a Parainfluenza Virus Vector Expressing the RSV G Protein Liang, Bo; Kabatova, Barbora; Kabat, Juraj Journal of Virology, Vol. 93, Issue 7 https://doi.org/10.1128/jvi.02043-18	journal	January 2019
Protective antigenic sites in respiratory syncytial virus G attachment protein outside the central conserved and cysteine noose domains Lee, Jeehyun; Klenow, Laura; Coyle, Elizabeth M. PLOS Pathogens, Vol. 14, Issue 8 https://doi.org/10.1371/journal.ppat.1007262	journal	August 2018
Role of Type I Interferon (IFN) in the Respiratory Syncytial Virus (RSV) Immune Response and Disease Severity Hijano, Diego R.; Vu, Luan D.; Kauvar, Lawrence M. Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.00566	journal	March 2019

Similar Records

Structure-Based Design and Antigenic Validation of Respiratory Syncytial Virus G Immunogens

Journal Article · Wed Mar 09 23:00:00 EST 2022 · Journal of Virology · OSTI ID:1903845

Decrease in Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) Enhanced Disease with RSV G Glycoprotein Peptide Immunization in BALB/c Mice

Journal Article · Sun Dec 22 23:00:00 EST 2013 · PLoS ONE · OSTI ID:1904890

An intranasal recombinant NDV-BRSV F_opt vaccine is safe and reduces lesion severity in a colostrum-deprived calf model of RSV infection

Journal Article · Wed Dec 28 23:00:00 EST 2022 · Scientific Reports · OSTI ID:2425583

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
antibodies
crystal structure
enzyme-linked immunoassays
epithelial cells
prophylaxis
respiratory infections
viral attachment
virus glycoproteins

Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies

Citation Formats

References (84)

Cited By (7)

Similar Records

Related Subjects