Decrease in Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) Enhanced Disease with RSV G Glycoprotein Peptide Immunization in BALB/c Mice

Rey, Gertrud U.; Miao, Congrong; Caidi, Hayat; Trivedi, Suvang U.; Harcourt, Jennifer L.; Tripp, Ralph A.; Anderson, Larry J.; Haynes, Lia M.

doi:10.1371/journal.pone.0083075

Decrease in Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) Enhanced Disease with RSV G Glycoprotein Peptide Immunization in BALB/c Mice

Journal Article · Mon Dec 23 00:00:00 EST 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0083075· OSTI ID:1904890

Rey, Gertrud U. ^[1]; Miao, Congrong ^[2]; Caidi, Hayat ^[2]; Trivedi, Suvang U. ^[2]; Harcourt, Jennifer L. ^[2]; Tripp, Ralph A. ^[3]; Anderson, Larry J. ^[4]; Haynes, Lia M. ^[2]

Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States); OSTI
Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States)
University of Georgia, Athens, GA (United States)
Emory University, Atlanta, GA (United States)

Respiratory syncytial virus (RSV) is a high priority target for vaccine development. One concern in RSV vaccine development is that a non-live virus vaccine would predispose for enhanced disease similar to that seen with the formalin inactivated RSV (FI-RSV) vaccine. Since a mAb specific to RSV G protein can reduce pulmonary inflammation and eosinophilia seen after RSV infection of FI-RSV vaccinated mice, we hypothesized that RSV G peptides that induce antibodies with similar reactivity may limit enhanced disease after subunit or other non-live RSV vaccines. In support of this hypothesis, we show that FI-RSV vaccinated mice administered RSV G peptide vaccines had a significant reduction in enhanced disease after RSV challenge. These data support the importance of RSV G during infection to RSV disease pathogenesis and suggest that use of appropriately designed G peptide vaccines to reduce the risk of enhanced disease with non-live RSV vaccines merits further study.

View Accepted Manuscript (DOE)

Research Organization:: Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: Centers for Disease Control and Prevention (CDC); National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1904890

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 12 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Enhanced Disease and Pulmonary Eosinophilia Associated with Formalin-Inactivated Respiratory Syncytial Virus Vaccination Are Linked to G Glycoprotein CX3C-CX3CR1 Interaction and Expression of Substance P Haynes, L. M.; Jones, L. P.; Barskey, A. Journal of Virology, Vol. 77, Issue 18 https://doi.org/10.1128/JVI.77.18.9831-9844.2003	journal	August 2003
Respiratory Syncytial Virus (RSV) G Glycoprotein Is Not Necessary for Vaccine-Enhanced Disease Induced by Immunization with Formalin-Inactivated RSV Johnson, Teresa R.; Teng, Michael N.; Collins, Peter L. Journal of Virology, Vol. 78, Issue 11 https://doi.org/10.1128/JVI.78.11.6024-6032.2004	journal	June 2004
Characterization of Recombinant Respiratory Syncytial Viruses with the Region Responsible for Type 2 T-Cell Responses and Pulmonary Eosinophilia Deleted from the Attachment (G) Protein Elliott, Matthew B.; Pryharski, Karin S.; Yu, Qingzhong Journal of Virology, Vol. 78, Issue 16 https://doi.org/10.1128/JVI.78.16.8446-8454.2004	journal	August 2004
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59 BASIC BIOLOGICAL SCIENCES
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mouse models
vaccination and immunization
vaccine development
vaccines
viral vaccines

Decrease in Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) Enhanced Disease with RSV G Glycoprotein Peptide Immunization in BALB/c Mice

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