Measurement of interaction force between nanoarrayed integrin {alpha}{sub v}{beta}{sub 3} and immobilized vitronectin on the cantilever tip

Journal Article · Sat Nov 03 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.08.084· OSTI ID:21032951

Lee, Minsu; Yang, Hyun-Kyu ^[1]; Park, Keun-Hyung ^[2]; Kang, Dong-Ku ^[3]; Chang, Soo-Ik ^[3]; Kang, In-Cheol ^[4]

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1. Chungbuk BIT Research-Oriented University Consortium, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
2. Department of Semiconductor Engineering, Research Institute of UbiquitousBio-Information Technology, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
3. Department of Biochemistry, Protein Chip Center, Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763 (Korea, Republic of)
4. Department of Biological Science, Hoseo University, Ansan 336-795 (Korea, Republic of)

Protein nanoarrays containing integrin {alpha}{sub v}{beta}{sub 3} or BSA were fabricated on ProLinker{sup TM}-coated Au surface by dip-pen nanolithography (DPN). An atomic force microscope (AFM) tip coated with ProLinker{sup TM} was modified by vitronectin. We measured the interaction force between nanoarrayed integrin {alpha}{sub v}{beta}{sub 3} or BSA and immobilized vitronectin on the cantilever tip by employing tethering-unbinding method. The unbinding force between integrin {alpha}{sub v}{beta}{sub 3} and vitronectin (1087 {+-} 62 pN) was much higher than that of between BSA and vitronectin (643 {+-} 74 pN). These results demonstrate that one can distinguish a specific protein interaction from non-specific interactions by means of force measurement on the molecular interactions between the nanoarrayed protein and its interacting protein on the AFM tip.

• OSTI ID: 21032951
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 362; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALBUMINS
ATOMIC FORCE MICROSCOPY
CALCIUM
CATTLE
MAGNESIUM
PH VALUE
PHOSPHATES